TB-500 Fragment (17-23)

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The Active Core of Thymosin Beta-4: An Introduction to TB-500 Fragment (17-23) 🔬

For researchers focused on the fundamental mechanisms of tissue repair, cell migration, and angiogenesis, the TB-500 Fragment (17-23) represents the critically active domain of the larger, well-studied peptide thymosin beta-4 (Tβ4). This synthetic 7-amino acid peptide corresponds to amino acid residues 17 through 23 of the native protein and carries the sequence Leu-Lys-Lys-Thr-Glu-Thr-Gln (LKKTETQ) .

The peptide segment (17)LKKTETQ(23) is widely recognized as the active site within the protein thymosin β4 responsible for actin binding, cell migration and wound healing . Studies suggest that this sequence occurs naturally, whereas TB-500 is a manufactured analog that incorporates this active region . The key ingredient of TB-500 products is often this peptide LKKTETQ with artificial acetylation of the N-terminus (Ac-LKKTETQ) .

TB-500 Fragment (17-23) is claimed to promote endothelial cell differentiation, angiogenesis in dermal tissues, keratinocyte migration, collagen deposition and decrease inflammation . By focusing research on this specific fragment, scientists can investigate the isolated effects of the actin-binding motif without the complexity of the full 43-amino acid protein, making it an invaluable tool for dissecting the molecular pathways underlying tissue regeneration and repair.

What is TB-500 Fragment (17-23)?

TB-500 Fragment (17-23) is a synthetic hepta-peptide with the sequence N-Acetyl-Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ) . Its molecular formula is consistently reported as C₃₈H₆₈N₁₀O₁₄ with a molecular weight of approximately 889.01 Da . It is identified by CAS number 885340-08-9 (free base) and CAS number 476014-70-7 for the fragment .

Its primary mechanisms of action, established through research on the parent thymosin beta-4 molecule and the isolated fragment, include:

1. Actin Binding and Cytoskeletal Regulation: The LKKTETQ motif is the critical domain responsible for binding to G-actin, the monomeric form of actin . By sequestering G-actin, this fragment regulates the pool of actin available for polymerization, a process fundamental to cell migration, adhesion, and morphological changes essential for wound healing .
2. Promotion of Cell Migration: Studies suggest that peptides corresponding to the Tβ4 amino acid sequence 17-23 stimulate the migration of key cells involved in tissue repair, including keratinocytes and endothelial cells . This enhanced migration is crucial for re-epithelialization of wounds and the formation of new blood vessels.
3. Angiogenesis Stimulation: TB-500 Fragment (17-23) promotes endothelial cell differentiation and angiogenesis in dermal tissues . By encouraging the formation of new capillary networks, it helps ensure adequate blood supply to regenerating tissues, facilitating the delivery of oxygen and nutrients essential for healing .
4. Collagen Deposition and Tissue Remodeling: Research indicates that this active fragment can influence collagen deposition, contributing to the structural reorganization and strengthening of healing tissues .
5. Anti-Inflammatory Effects: The fragment has been associated with a decrease in inflammation , creating a more favorable environment for tissue repair and regeneration.

🔬 Primary Research Focuses and Applications

The TB-500 Fragment (17-23) is primarily utilized in scientific research to investigate a broad spectrum of regenerative and cellular processes:

• Wound Healing and Tissue Regeneration Research: As the active domain responsible for many of thymosin beta-4's healing properties, this fragment is extensively studied for its ability to accelerate wound closure, promote re-epithelialization, and improve the quality of healed tissue .

• Angiogenesis and Vascular Research: The fragment's ability to promote endothelial cell differentiation and new blood vessel formation makes it a valuable tool for studying therapeutic angiogenesis in models of ischemia, wound repair, and tissue engineering .

• Cell Migration and Cytoskeletal Dynamics: Researchers use this peptide to investigate the fundamental mechanisms of cell motility, particularly how actin-binding proteins influence the cytoskeleton to drive cellular movement .

• Musculoskeletal and Tendon Repair Research: Given its role in promoting cell migration and collagen deposition, the fragment is studied in preclinical models of tendon, ligament, and muscle injury repair .

• Comparative Studies with Full-Length Thymosin Beta-4: The fragment serves as a precise tool to dissect which specific biological activities of the full 43-amino acid Tβ4 protein are attributable to its actin-binding motif (17-23), versus other regions of the molecule .

🧪 Product Specifications & Technical Data

At Buy Peptides Online USA, we ensure your research is built on a foundation of quality and consistency. This peptide is manufactured to rigorous laboratory standards and verified through advanced analytical methods.

✅ Why Choose Buy Peptides Online USA for Your Research?

Sourcing reliable research compounds is critical for reproducible, high-impact science. Here is why discerning researchers across the USA trust us:

• Verified Purity & Potency: We provide access to Certificates of Analysis (COA) and rely on advanced analytical methods like High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) to confirm the identity and purity (≥98%) of every batch . This commitment ensures the integrity of your experimental data.
• Research-Focused Integrity: We strictly adhere to research-use-only (RUO) guidelines. Our product descriptions are factual, focusing on chemical properties and cited research applications, never unverified human-use claims. This product is not for diagnostic or therapeutic use .
• Fast & Discreet USA Shipping: As a USA-based supplier, we offer fast, reliable shipping across the United States with discreet packaging to ensure your order arrives safely, professionally, and on time for your critical research timelines. Enjoy free shipping on orders over $300.
• Expert Support & Authenticity Guarantee: Our team provides technical consultation to support your work. We stand behind the authenticity and quality of all our products, offering a product replacement service and clear complaint handling.

📝 Handling, Storage, & Important Safety Information

• Critical Storage Information: Upon receipt, store the lyophilized powder at -20°C (-4°F) or below, desiccated, protected from light and moisture. It is stable for >3 years under these conditions . Although stable at room temperature for a few weeks during ordinary shipping, long-term storage requires freezing .
• Handling for Research Use: This product is for laboratory use only by qualified professionals. Before opening, allow the vial to warm to room temperature and briefly centrifuge to concentrate material at the bottom. The peptide is soluble in DMSO ; subsequent dilutions should be made into appropriate aqueous buffers for biological assays. Gently invert to mix; do not vortex.
• Stability Considerations: Once reconstituted, for short-term storage (days to weeks), keep at 0-4°C. For long-term storage, freeze at -20°C. Avoid repeated freeze-thaw cycles at all costs, as this can degrade the peptide .
• Safety Considerations: TB-500 Fragment (17-23) is a potent, bioactive peptide for research use only. Always wear appropriate personal protective equipment (PPE), including lab coats, safety glasses, and nitrile gloves. Work in a laminar flow hood or well-ventilated area.
• Warning: This product is not for human or veterinary use. It is not a drug, food, or cosmetic. Not for human consumption or diagnostic use . For laboratory research only.

❓ Frequently Asked Questions (FAQs)

Q: What is the TB-500 Fragment (17-23)?

A: It is a synthetic 7-amino acid peptide (Ac-LKKTETQ) representing the active site of the naturally occurring protein thymosin beta-4. This specific sequence (residues 17-23) is responsible for actin binding, cell migration, and wound healing activities .

Q: How does this fragment differ from the full-length TB-500 (43aa) peptide?

A: The full-length TB-500 (43aa) is the complete thymosin beta-4 protein . The TB-500 Fragment (17-23) is just the active core domain. By using this fragment, researchers can isolate and study the specific mechanisms related to actin binding and cell migration without the influence of other regions of the full protein .

Q: What are the key research applications for this fragment?

A: It is primarily used in research into wound healing, tissue regeneration, angiogenesis (new blood vessel formation), cell migration, and cytoskeletal dynamics .

Q: What is the molecular weight and formula of TB-500 Fragment (17-23)?

A: It has a molecular formula of C₃₈H₆₈N₁₀O₁₄ and a molecular weight of approximately 889.01 Da . Its CAS number is 885340-08-9 .

Q: What is the significance of the N-terminal acetylation?

A: The N-terminal acetylation (Ac-LKKTETQ) is a common modification that enhances the peptide's stability and resistance to enzymatic degradation .

Q: How do you verify the purity and identity of this peptide?

A: We ensure every batch is rigorously analyzed using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) . HPLC confirms a purity level of ≥98% , while Mass Spectrometry verifies the precise molecular weight (~889 Da) , ensuring the correct peptide structure and identity. Certificates of Analysis (COA) are available upon request.

Q: What are the critical storage conditions for TB-500 Fragment (17-23)?

A: Store the lyophilized powder at -20°C (-4°F) or below, desiccated and protected from light and moisture; it is stable for >3 years under these conditions . Once reconstituted, for short-term storage, keep at 0-4°C . Avoid repeated freeze-thaw cycles.

Q: Is this peptide prohibited in sports research?

A: Yes, researchers should be aware that thymosin beta-4 and its synthetic analogs, including fragments, are considered prohibited substances under the general category of peptide hormones and growth factors on the World Anti-Doping Agency (WADA) Prohibited List . It is intended for legitimate scientific research only.

Ready to Advance Your Research? 🚀

Equip your laboratory with the active core peptide for studying the fundamental mechanisms of cell migration, angiogenesis, and tissue repair. Order the high-purity TB-500 Fragment (17-23) today from Buy Peptides Online USA. Benefit from our fast USA shipping, secure payments (including crypto), and the confidence that comes from rigorously tested, research-grade compounds. For any questions about this product or your order, our support team is here to provide technical consultation.