Humanin Peptide

Humanin Peptide (Mitochondrial-Derived Peptide)

Welcome to buypeptidesonlineusa.com, your premier domestic source for high-purity research peptides. Our Humanin is a synthetic 24-amino acid peptide, identical to the endogenous mitochondrial-derived peptide (MDP) first discovered for its neuroprotective properties. It is meticulously manufactured for advanced investigations into cellular stress responses, apoptosis, neurodegenerative disease models (including Alzheimer's disease), metabolic regulation, and the biology of aging. As a key modulator of cell survival pathways, Humanin is a foundational tool for researchers exploring the frontiers of cytoprotection and mitochondrial signaling. We ensure your critical research is supported by uncompromising quality, transparency, and rapid fulfillment.

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⚠️ Important Research Compliance Notice

This product is intended strictly for laboratory research use only and is not for human consumption or veterinary use. This product is not a medicine, drug, or medical device. It has not been evaluated by the FDA. This peptide is sold for investigational purposes by qualified researchers and professionals only.

🔬 What is Humanin Peptide?

Humanin (HN) is a 24-amino acid peptide encoded within the mitochondrial genome, specifically by the mitochondrial 16S ribosomal RNA gene (MT-RNR2) . It was first discovered in 2001 in an unaffected region of an Alzheimer's disease patient's brain, where it was found to protect neurons from the toxic effects of amyloid-beta (Aβ) and mutant genes associated with familial Alzheimer's disease . Since its discovery, Humanin has been recognized as the founding member of a larger family of mitochondrial-derived peptides (MDPs) and has emerged as a pleiotropic cytoprotective factor with a wide range of biological activities.

The peptide sequence is: Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala . Key structural features include a central hydrophobic region and a C-terminal domain crucial for its receptor binding and activity.

Humanin's mechanisms of action are multifaceted and involve both intracellular and extracellular pathways :

• Intracellularly, it can bind to and inhibit pro-apoptotic proteins like Bax, preventing mitochondrial outer membrane permeabilization and the release of cytochrome c, thereby blocking the intrinsic pathway of apoptosis .
• Extracellularly, it acts as a signaling molecule by binding to a putative trimeric receptor complex involving CNTF, WSX-1, and gp130, activating downstream survival pathways such as JAK/STAT .
• It also possesses potent anti-oxidant properties, reducing reactive oxygen species (ROS) and protecting against oxidative stress .

✅ Why Choose Buy Peptides Online USA for Your Research?

When you need to buy Humanin peptide online, quality and consistency are non-negotiable. Here is why discerning researchers choose us:

• Verified Purity: We provide access to independent third-party Certificate of Analysis (COA) documents, confirming purity via High-Performance Liquid Chromatography (HPLC) exceeding 98% and identity via Mass Spectrometry (MS) .
• Optimal Storage & Handling: Our Humanin is shipped in lyophilized form. For long-term stability, store desiccated at -20°C or below, protected from light and moisture. Lyophilized Humanin is stable for up to 36 months under these conditions .
• Domestic Shipping: As a USA-based supplier, we offer fast, reliable shipping. Orders typically arrive within 2-5 business days in discreet, temperature-controlled packaging.
• Research-Focused: We make no medical claims. Our service is built on integrity, providing accurately labeled products for legitimate scientific inquiry.
• Secure Transactions: Enjoy peace of mind with secure checkout and multiple payment options, including credit cards and cryptocurrency.

🔎 Product Specifications & Technical Data

To ensure your research parameters are met with precision, here are the verified technical specifications for our Humanin peptide. Purity is a critical determinant of research accuracy, and our product meets the high standards expected by the scientific community .

🧪 Key Research Applications & Focus Areas

Humanin's diverse cytoprotective functions make it a powerful tool across a wide spectrum of biomedical research .

• 🧠 Neurodegenerative Disease Research (Alzheimer's Disease): This is the foundational application of Humanin. It is extensively used to model neuroprotection against amyloid-beta (Aβ) toxicity . Researchers study its ability to improve hippocampal function, attenuate oxidative stress, and counteract the molecular effects of Alzheimer's-related insults, making it a key probe for understanding disease mechanisms and evaluating potential therapeutic strategies .

• 🔬 Apoptosis & Cell Survival Pathways: Humanin is a valuable tool for studying the intrinsic (mitochondrial) pathway of apoptosis. By binding to and inhibiting Bax, it blocks mitochondrial permeability and subsequent caspase activation . Researchers use it to investigate the regulation of programmed cell death in various models of cellular stress.

• ⚖️ Metabolic & Endocrine Research: Humanin plays a significant role in metabolism. It has been shown to improve insulin sensitivity and beta-cell survival, making it relevant for studying diabetes and metabolic disorders . It is also an autophagy inducer, promoting cell survival under metabolic stress .

• 🕰️ Aging & Longevity Research: As a mitochondrial-derived peptide, Humanin is a critical biomarker and potential regulator of healthspan and lifespan . Circulating Humanin levels decline with age, and its age-related decrease is linked to cognitive impairment and increased risk of neurodegeneration . It is used to study the fundamental biology of aging and the role of mitochondrial signaling in the aging process.

• ❤️ Cardiovascular Research: Humanin exhibits protective effects in the cardiovascular system. It protects cardiomyocytes from oxidative stress damage, reduces reactive oxygen species (ROS) production, and may help prevent myocardial fibrosis, making it a tool for studying aging-related cardiovascular diseases .

• 🛡️ Inflammation Research: Humanin possesses anti-inflammatory properties, modulating inflammatory pathways and contributing to its broad cytoprotective profile .

🔬 How Does Humanin Work? (Mechanism of Action)

The mechanism of Humanin is complex and involves multiple complementary pathways :

• Inhibition of Intrinsic Apoptosis: Humanin directly interacts with the pro-apoptotic protein Bax in the cytoplasm, preventing its translocation to the mitochondria. This stops Bax from forming pores in the mitochondrial membrane, thereby inhibiting the release of cytochrome c and the subsequent activation of caspases .
• Activation of Cell Survival Signaling: Extracellular Humanin binds to a specific receptor complex (CNTFRα/WSX-1/gp130). This activates the JAK/STAT signaling pathway, leading to the expression of genes that promote cell survival and inhibit cell death .
• Reduction of Oxidative Stress: Humanin reduces the production of reactive oxygen species (ROS) and increases the expression of antioxidant defense system proteins, protecting cells from oxidative damage .
• Modulation of Autophagy: Humanin acts as an autophagy inducer, helping cells clear damaged organelles and proteins, which is crucial for maintaining cellular health under metabolic and oxidative stress .

📝 Handling, Storage, and Preparation for Research

Proper handling is critical to maintaining peptide integrity and ensuring experimental validity .

• Receiving & Storage: Upon receipt, store the lyophilized vial in a freezer at -20°C (-4°F) or below. Protect from light and moisture. Lyophilized Humanin is stable for up to 36 months under these conditions.
• Reconstitution: To prepare stock solutions, Humanin is soluble in water. It is recommended to reconstitute in sterile 18MΩ-cm H₂O at a concentration of at least 100 µg/ml . Allow the peptide to warm to room temperature in a desiccator before opening. Gently swirl or invert to dissolve; do not vortex.
• Dosage Considerations:
  • In vitro: Concentrations in the low micromolar range (e.g., 1-10 µM) are typically used in cell culture experiments to study neuroprotection or anti-apoptotic effects .
  • In vivo (Rodent): Studies often use the potent analog HNG at doses such as 4 mg/kg body weight administered intraperitoneally (IP) twice weekly to achieve cognitive improvements in aged mouse models .
• Preventing Adsorption: At low concentrations, peptides can adsorb to plastic surfaces. To prevent this, use low-protein-binding tubes and consider adding a carrier protein (e.g., 0.1% BSA) to the dilution buffer.
• Safety & Handling: Always use personal protective equipment (PPE) such as gloves and safety goggles when handling. Work in a laminar flow hood or well-ventilated area. Dispose of all waste in appropriate biohazard containers.

❓ Frequently Asked Questions (FAQ)

Q1: What is Humanin and where does it come from?

A: Humanin is a 24-amino acid peptide that is encoded in the mitochondrial genome (MT-RNR2 gene) . It is a naturally occurring mitochondrial-derived peptide (MDP) that is synthesized in the laboratory for research purposes.

Q2: What are the primary research applications for Humanin?

A: Its primary applications are in neuroprotection (especially Alzheimer's disease research) , studying apoptosis and cell survival, metabolic regulation (diabetes, autophagy) , and the biology of aging and longevity .

Q3: How do I verify the quality of your Humanin?

A: We are committed to transparency. Our product listings provide access to a third-party Certificate of Analysis (COA) . This document validates the compound's identity via Mass Spectrometry (MS) and its purity via High-Performance Liquid Chromatography (HPLC), which is ≥98% .

Q4: Are there more potent analogs of Humanin used in research?

A: Yes. [Gly14]-Humanin (HNG) , where the serine at position 14 is replaced with glycine, is a widely studied analog that exhibits significantly enhanced cytoprotective activity, being up to 1000 times more potent than the native peptide . It is used to amplify the effects seen with native Humanin in disease models.

Q5: What is the correct way to store reconstituted Humanin?

A: For short-term use (2-7 days), store the solution at 4°C. For longer storage, it is essential to aliquot the solution into single-use vials and freeze at -20°C or below. Adding a carrier protein like 0.1% BSA is highly recommended to maintain stability. Avoid repeated freeze-thaw cycles .

📦 Our Services & Guarantees

• 24/7 Customer Support: Our team is available to answer your pre- and post-sales questions.
• Authenticity Guarantee: We guarantee that you receive the exact product described, with full documentation and lot matching.
• Product Replacement: In the unlikely event of damage during shipping, we have a clear process for claims and replacement.
• Technical Consultation: Contact us for assistance with solubility or handling protocols.

🚀 How to Buy Humanin Peptide Online

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1. Browse & Select: Choose the quantity you need (e.g., 1mg, 5mg vial).
2. Add to Cart: Proceed to our secure checkout.
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4. Shipping: Orders are processed within 24 hours on business days and shipped in discreet, temperature-controlled packaging. Domestic orders typically arrive in 2-5 days.

Ready to advance your research into cell survival and mitochondrial signaling? Add high-purity Humanin peptide to your cart today.