Fragment, CJC-1295 (no DAC), Ipamorelin (Blend)

🔬 Introduction: The Lipolytic-GHRH-GHRP Triad

Welcome to Buy Peptides Online USA, the trusted source for high-purity research peptides online. Our Fragment, CJC-1295 (no DAC), Ipamorelin Blend represents the first true metabolic+secretagogue triad available in a single, pre-formulated vial.

This blend unites three mechanistically distinct peptide families :

1. HGH Fragment 176-191: A 17-amino acid C-terminal fragment of human Growth Hormone. Unlike full-length rhGH, this fragment exerts selective lipolytic activity via direct stimulation of β3-adrenergic receptors in adipocytes, completely isolated from IGF-1-mediated mitogenic effects .
2. CJC-1295 (no DAC) / Mod GRF 1-29: A tetrasubstituted GHRH analog. Stimulates pituitary somatotrophs via the cAMP/PKA/CREB pathway. Its short half-life (~30 min) models physiological pulsatility .
3. Ipamorelin: A highly selective GHSR-1a agonist (GHRP). Amplifies the GH pulse initiated by CJC-1295 while demonstrating negligible effects on prolactin, ACTH, or cortisol in preclinical models .

Why this triad? This is the only commercially available blend that allows researchers to investigate three distinct axes simultaneously :

• Adipose tissue lipolysis (HGH Frag)
• Pituitary GH synthesis (CJC-1295)
• Somatostatin antagonism (Ipamorelin)

This product is strictly for in vitro and laboratory research use only.

✅ Why Choose This Triple Blend from Buy Peptides Online USA?

The peptide research market is saturated with undefined "research liquids" and binary blends. We differentiate through forensic transparency and triple-pathway precision.

Why does this matter? In metabolic research, impurities and incorrect molar ratios introduce confounding variables that invalidate lipolysis assays, cAMP quantification, and receptor binding studies. Our blend ensures your data reflects the biology, not the synthesis artifacts.

🧪 What Is the Fragment, CJC-1295 no DAC & Ipamorelin Blend? (Mechanistic Deep-Dive)

🔹 Component A: HGH Fragment 176-191 – The Selective Lipolytic Agent

• Full Sequence: NH₂-Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe-COOH
• Molecular Formula: C₇₈H₁₂₁N₂₃O₂₂S₂
• Molecular Weight: ~1768.0 g/mol
• CAS Number: 126752-37-8 (Research Grade)
• Synonyms: AOD-9604 (Parent Compound), hGH 177-191, Lipolytic Fragment

🔬 Mechanism of Action:
HGH Fragment 176-191 is a C-terminal peptide derived from the loop between amino acids 176 and 191 of the human Growth Hormone molecule. Unlike full-length rhGH, it does not bind the GH receptor dimer and therefore does not stimulate IGF-1 production or cell proliferation .

Lipolytic Pathway:

1. β3-Adrenergic Receptor Activation: The fragment directly stimulates adipocyte β3-ARs.
2. HSL Activation: Hormone-Sensitive Lipase is phosphorylated.
3. Triglyceride Hydrolysis: Adipose triglyceride is broken down into free fatty acids and glycerol.
4. Mitochondrial Oxidation: FAs are directed toward β-oxidation rather than re-esterification .

Research Advantage: This fragment allows researchers to model fat mass reduction in isolation, without the confounding anabolic/mitogenic signals of full-length HGH or IGF-1 .

🔹 Component B: CJC-1295 (no DAC) / Mod GRF 1-29 – The GHRH Analog

• Full Sequence (Single Letter): YaDAIFTQSYRKVLAQLSARKLLQDILSR-NH₂
• Molecular Formula: C₁₅₂H₂₅₂N₄₄O₄₂
• Molecular Weight: ~3367.97 g/mol
• CAS Number: 863288-34-0
• Synonyms: Modified GRF (1-29), CJC-1295 without DAC, Tetrasubstituted GRF

🔬 Mechanism of Action:
This is a 29-amino acid, tetrasubstituted GHRH analog. Modifications at positions 2 (D-Ala), 8 (Gln), 15 (Ala), and 27 (Leu) confer resistance to dipeptidyl peptidase-4 (DPP-4) degradation, extending the half-life to ~30 minutes . It acts as a full agonist at the GHRH receptor on pituitary somatotrophs, stimulating GH synthesis and release .

🔹 Component C: Ipamorelin – The Selective GHRP

• Sequence: Aib-His-D-2-Nal-D-Phe-Lys-NH₂
• Molecular Formula: C₃₈H₄₉N₉O₅
• Molecular Weight: ~711.9 g/mol
• CAS Number: 170851-70-4
• Synonyms: Selective Ghrelin Mimetic, GHRP-1 Analog

🔬 Mechanism of Action:
Ipamorelin is a pentapeptide and a highly selective agonist at the GHSR-1a receptor. It mimics ghrelin's action, suppressing somatostatin tone and amplifying the GH pulse initiated by GHRH. Its key advantage is selectivity: it demonstrates minimal cross-reactivity with other pituitary axes (ACTH, Prolactin, Cortisol) .

📊 Peptide Specifications & Technical Data

Verified via Third-Party HPLC-MS. COA available upon request.

🔬 Primary Research Applications & Investigational Focus

This product is for laboratory research only. The following outlines mechanisms reported in peer-reviewed, non-human studies.

🔥 1. Adipose Tissue Lipolysis & Metabolic Flux (HGH Fragment-Dominant)

The signature application of this triad. HGH Fragment 176-191 allows researchers to model β3-adrenergic lipolysis in primary adipocyte cultures or 3T3-L1 cell lines. When combined with CJC/Ipamorelin, investigators can study the interplay between GH pulsatility and fat oxidation kinetics without the confounding anabolic drive of full-length HGH .

📈 2. Pulsatile GH Secretion & Somatotroph Sensitivity

CJC-1295 no DAC provides the GHRH signal, while Ipamorelin suppresses somatostatin. This blend is optimal for dose-response cAMP assays and GH release ELISA time-course studies. The absence of DAC ensures receptors are not desensitized by continuous occupancy, preserving physiological relevance .

⚖️ 3. Body Composition & Metabolic Homeostasis Models

This blend is extensively utilized in rodent models of caloric restriction, diet-induced obesity (DIO) , and sarcopenic obesity. Researchers can investigate the synergistic effects of GH pulse amplification (CJC/Ipa) and direct adipocyte stimulation (Fragment) on lean mass retention and visceral fat reduction .

🧬 4. GHSR-1a Signal Bias & Receptor Cross-Talk

Ipamorelin is a biased agonist at the ghrelin receptor. This blend allows researchers to parse β-arrestin recruitment vs. Gq/11 signaling in the presence of concurrent GHRH stimulation. This is critical for understanding receptor desensitization and ligand-biased trafficking .

💤 5. Sleep Architecture & Nocturnal GH Pulses

GH secretion is tightly coupled to slow-wave sleep (SWS) . This blend is employed in circadian rhythm research to model how augmented GH pulses influence sleep architecture, neural repair, and HPA axis regulation .

🧪 Quality Verification: HPLC, Mass Spectrometry & COA

We do not rely on spot-checking. Every batch of our Fragment, CJC-1295 (no DAC), Ipamorelin Blend undergoes rigorous analytical chemistry validation per USP/EP guidelines .

1. High Performance Liquid Chromatography (HPLC):

   • Method: Reverse-Phase C18 Column, Gradient Elution.
   • Criteria: Purity ≥99% per component. Absence of deletion sequences and oxidation products .
   • System Suitability: Theoretical plates ≥5000, tailing factor ≤2.0 (per GB/T 38163-2019) .

2. Mass Spectrometry (MS):

   • Method: Electrospray Ionization (ESI-MS).
   • Criteria: Confirmed molecular weights: 1768.0 Da, 3367.97 Da, 711.9 Da. This confirms full-length, correctly folded peptide chains.

3. Endotoxin Analysis (LAL Test):

   • Method: Kinetic Chromogenic LAL.
   • Criteria: <0.05 EU/mg. Significantly exceeds industry standard (≤5 EU/mg), ensuring compatibility with sensitive primary adipocyte and pituitary cell cultures .

4. Certificate of Analysis (COA):
   A batch-specific COA is available immediately upon request. This document includes:

   • Lot Number & Manufacturing Date
   • Retention Time & Purity Percentage
   • MS Spectrum & Molecular Weight Confirmation
   • Endotoxin Value & Appearance Verification

❄️ Handling, Storage & Reconstitution Protocol

For Laboratory Use Only – Not for Human Consumption.

📦 Long-Term Storage (Lyophilized)

• Temperature: Store at -20°C (-4°F) in a manual-defrost freezer .
• Stability: Stable for 12 months at -20°C. Stable at room temperature for up to 90 days, but refrigeration is recommended for archival storage .
• Environment: Keep desiccated. Protect from UV light and humidity .

💧 Reconstitution Instructions (Standard Laboratory Protocol)

Based on standard research protocols for triple blends :

1. Equilibrate: Allow the vial to reach room temperature in a desiccator to prevent moisture condensation.
2. Inject: Using a sterile syringe, inject 3.0 mL of Bacteriostatic Water (BAC) slowly against the inner glass wall.
   • Resulting Concentration: 5 mg/mL total peptide (1.67 mg/mL each of Frag, CJC, and Ipa).
3. Swirl: Do NOT vortex or shake vigorously. Shaking introduces oxygen radicals and shears the peptide bonds. Gently swirl until the liquid is clear .
4. Note: HGH Fragment 176-191 may require gentle swirling to fully dissolve; it is readily soluble in BAC water.

🧊 Post-Reconstitution Handling

• Storage: 2-8°C (Refrigerator). Do not freeze .
• Duration: Use within 21-28 days. Discard if cloudiness or particulate matter persists .
• ⚠️ Critical Alert: Freezing reconstituted peptides causes ice crystal shearing, permanently destroying the tertiary structure. Do not refreeze .

📦 Packaging, Shipping & Buyer Services

We engineer the unboxing experience to match the precision of our analytics.

• 📐 Discreet & Secure Packaging: Vials are seated in medical-grade foam inside rigid, opaque mailers.
• 🌡️ Thermal Integrity: Seasonal cold-pack inserts included to maintain ≤4°C during summer transit .
• 🇺🇸 USA Dispatch: Shipped from domestic logistics hubs. Transit time: 2-5 business days.
• 🛡️ Buyer Guarantees:
  • Authenticity Guarantee: Direct sourcing from cGMP-compliant, FDA-registered facilities.
  • 24/7 Technical Consultation: Lab handling queries answered via email support.
  • Product Replacement: Immediate replacement for damaged or compromised vials (photographic proof required).
  • Complaint Handling: Dedicated resolution team for shipping discrepancies.

❓ Frequently Asked Questions (FAQ)

Q: What exactly is "Fragment" in this blend?

A: "Fragment" universally refers to HGH Fragment 176-191. It is a 17-amino acid peptide derived from the C-terminus of human Growth Hormone. It is not AOD-9604 (which is 177-191), but the slightly longer, more stable 176-191 variant widely used in lipolysis research .

Q: Why blend HGH Fragment with CJC and Ipamorelin?

A: This creates a comprehensive metabolic research tool. CJC/Ipamorelin amplifies endogenous GH pulsatility (which has indirect lipolytic effects via IGF-1), while HGH Fragment directly stimulates β3-adrenergic lipolysis in adipocytes. This allows researchers to model central + peripheral fat metabolism pathways simultaneously .

Q: What is the purity of this blend?

A: ≥99% per component. This is verified via HPLC (area normalization) and ESI-MS for each batch. We do not sell "≥98%" material .

Q: What is the advantage of CJC-1295 "no DAC" over "with DAC"?

A: CJC-1295 with DAC has a half-life of ~8 days and causes continuous receptor occupancy, leading to somatotroph desensitization. CJC-1295 no DAC has a half-life of ~30 minutes, modeling physiological pulsatility and allowing for receptor recovery between pulses .

Q: Does HGH Fragment 176-191 stimulate IGF-1?

A: No. This is the critical distinction. Unlike full-length HGH, Fragment 176-191 does not bind the GH receptor dimer and therefore does not stimulate hepatic IGF-1 production or mitogenic signaling. Its effects are confined to adipocyte metabolism .

Q: How long is the shelf life after reconstitution?

A: When reconstituted with Bacteriostatic Water (0.9% benzyl alcohol), the solution is stable for 21-28 days at 2-8°C. Sterile water without preservatives shortens this window significantly .

Q: Do you provide dosing instructions?

A: Absolutely not. As a Research Use Only (RUO) supplier, we strictly prohibit providing protocols for human or animal administration. We supply the raw material; determining molar concentrations for specific in vitro assays (e.g., 10nM vs. 100nM) is the responsibility of the qualified researcher .

⚖️ Legal & Compliance Disclaimer

Research Use Only (RUO)
This product is sold exclusively for laboratory research and educational purposes. It is NOT a drug, food, dietary supplement, or cosmetic. This product has not been approved by the U.S. Food and Drug Administration (FDA) . Bodily introduction of any kind, including human or veterinary consumption, injection, or topical application, is strictly prohibited and illegal.

By purchasing from Buy Peptides Online USA, you certify that you are a qualified researcher, scientist, or laboratory professional and will handle this material in compliance with all applicable local, state, and federal regulations, including institutional biosafety protocols .

⭐ Transactional Close: Why Principal Investigators Buy Here

Ready to eliminate confounding variables from your metabolic and somatotroph signaling assays? Here is why top labs buy peptides online from Buy Peptides Online USA:

• 💰 Superior Value: 15mg total peptide mass (5mg Frag + 5mg CJC + 5mg Ipa) at a competitive price point versus buying three individual vials.
• ⚡ Velocity: Same-day processing on orders placed before 2:00 PM EST.
• 🔒 Secure Checkout: Accepting major credit cards and cryptocurrency (BTC/ETH/USDT) for institutional privacy and discretion.
• 📞 Post-Sale Support: Direct access for technical consultation regarding solubility, storage stability, or analytical data interpretation.

📄 Request a COA: Use our contact form with your Order Number or Batch Lot Number to receive the full analytical package, including HPLC chromatograms and MS spectra.