PE-22-28 Peptide

PE-22-28 Peptide (Mini-Spadin, TREK-1 Inhibitor)

Welcome to buypeptidesonlineusa.com, your premier domestic source for high-purity research peptides. Our PE-22-28 Peptide, also known as Mini-Spadin, is a synthetic 7-amino acid peptide derived from the natural sortilin-based peptide Spadin. It is a potent and specific inhibitor of the TREK-1 potassium channel, meticulously manufactured for advanced investigations into neuroprotection, post-stroke recovery, major depressive disorder (MDD), neurogenesis, and synaptic plasticity. As a promising lead compound with demonstrated efficacy in preclinical models, PE-22-28 is an essential tool for researchers exploring innovative therapeutic targets in neurology and psychiatry. We ensure your critical research is supported by uncompromising quality, transparency, and rapid fulfillment.

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⚠️ Important Research Compliance Notice

This product is intended strictly for laboratory research use only and is not for human consumption or veterinary use. This product is not a medicine, drug, or medical device. It has not been evaluated by the FDA. This peptide is sold for investigational purposes by qualified researchers and professionals only.

🔬 What is PE-22-28 Peptide?

PE-22-28 (also known as Mini-Spadin) is a synthetic heptapeptide with the amino acid sequence Gly-Val-Ser-Trp-Gly-Leu-Arg (GVSWGLR) . It is derived from a longer, naturally occurring peptide called Spadin (PE 12-28), which is itself generated by the post-translational cleavage of the sortilin protein .

The development of PE-22-28 represents a significant advancement in the search for novel treatments for neurological and psychiatric conditions. While Spadin was first identified as a specific blocker of the TREK-1 potassium channel with rapid antidepressant effects, its action in the body was short-lived. Researchers synthesized shorter analogs of Spadin to improve its efficacy and bioavailability. Through behavioral and electrophysiological screening, PE-22-28 (Mini-Spadin) was identified as the lead peptide, demonstrating greater potency and a longer duration of action than its parent compound .

Its key mechanism of action is the potent and specific inhibition of the TREK-1 channel (KCNK2), a two-pore domain potassium channel. TREK-1 is a well-validated target for depression and stroke because its activity is linked to neuronal excitability and mood regulation. By blocking this channel, PE-22-28 modulates neuronal activity, leading to downstream effects that include:

• Increased Neurogenesis: PE-22-28 treatment has been shown to increase the birth of new neurons (neurogenesis), particularly in the hippocampus, a brain region critical for learning, memory, and mood .
• Enhanced Synaptogenesis: It promotes the formation of new synapses (synaptogenesis), strengthening connections between neurons .
• Neuroprotection: It prevents the degeneration of vulnerable neurons, such as dopaminergic neurons in the substantia nigra, after an ischemic event .

✅ Why Choose Buy Peptides Online USA for Your Research?

When you need to buy PE-22-28 peptide online, quality and consistency are non-negotiable. Here is why discerning researchers choose us:

• Verified High Purity: We provide access to independent third-party Certificate of Analysis (COA) documents, confirming purity via High-Performance Liquid Chromatography (HPLC) at ≥98% and identity via Mass Spectrometry (MS) .
• Authentic Structure: Our peptide is the synthetic heptapeptide (GVSWGLR) with the correct molecular weight, matching the compound validated in groundbreaking pre-clinical studies from Sorbonne University .
• Potent Biological Activity: As a specific TREK-1 inhibitor with an IC50 value of 0.12 nM, our high-purity PE-22-28 ensures you are working with a fully active molecule .
• Optimal Storage & Handling: Our PE-22-28 is shipped in lyophilized (freeze-dried) powder form. For long-term stability, store desiccated at -20°C or below . In lyophilized form, it is stable for up to 3 years .
• Domestic Shipping: As a USA-based supplier, we offer fast, reliable shipping. Orders typically arrive within 2-5 business days in discreet packaging.
• Research-Focused: We make no medical claims. Our service is built on integrity, providing accurately labeled products for legitimate scientific inquiry.

🔎 Product Specifications & Technical Data

To ensure your research parameters are met with precision, here are the verified technical specifications for our PE-22-28 peptide. Purity, identity, and activity are critical determinants of research accuracy, and our product meets the high standards expected by the scientific community .

🧪 Key Research Applications & Focus Areas

PE-22-28's unique mechanism as a potent and specific TREK-1 inhibitor makes it a powerful tool across a spectrum of cutting-edge neuroscience research, as validated by studies from Sorbonne University .

• 🧠 Post-Stroke Recovery (Ischemia) Research: This is a primary and extensively studied application of PE-22-28. In a mouse model of stroke (middle cerebral artery occlusion, MCAO), PE-22-28 treatment demonstrated significant neuroprotective properties. It reduced the mortality rate, prevented weight loss, and lessened motor and cognitive dysfunctions induced by ischemia. The treatment also prevented the degeneration of dopaminergic neurons in the substantia nigra, a region often affected by stroke .

• 🛡️ Depression & Antidepressant Research: PE-22-28 was developed from the natural antidepressant peptide Spadin to improve its efficacy and bioavailability. It has been shown to block the TREK-1 potassium channel, a well-validated target for depression. In animal models (Forced Swimming Test, FST; Novelty-Induced Feeding Suppression, NSF), PE-22-28 effectively prevented the development of depressive behavior following trauma, opening possibilities for innovative treatments with potentially faster action and fewer side effects than current therapies .

• 🧬 Neurogenesis & Synaptic Plasticity: PE-22-28 treatment has been shown to robustly increase both neurogenesis (the birth of new neurons) and synaptogenesis (the formation of new synapses), particularly in the hippocampus. This makes it an invaluable tool for studying the cellular mechanisms underlying learning, memory, and recovery from brain injury .

• 🔄 Anxiety & Stress-Related Disorders: Given the role of TREK-1 channels in regulating neuronal excitability in brain regions involved in fear and anxiety (like the amygdala), PE-22-28 is a promising tool for researching anxiety disorders and stress resilience.

📝 Handling, Storage, and Preparation for Research

Proper handling is critical to maintaining PE-22-28's integrity and ensuring experimental validity .

• Receiving & Storage: Upon receipt, store the lyophilized vial in a freezer at -20°C (-4°F) or below. Protect from light and moisture. Lyophilized peptide is stable for up to 3 years under these conditions .
• Reconstitution: To prepare stock solutions, allow the vial to reach room temperature in a desiccator. PE-22-28 is soluble in water . Gently swirl or invert to dissolve; do not vortex to prevent denaturation.
• Aliquoting & Storage Post-Reconstitution: It is highly recommended to prepare single-use aliquots immediately after reconstitution to avoid the activity loss caused by repeated freeze-thaw cycles .
  • For short-term use, store aliquots at 2-8°C.
  • For long-term storage, store aliquots at -80°C for up to 6 months, or at -20°C for up to 1 month .
• Dosage Considerations (for reference):
  • In vivo (Rodent): Preclinical studies validating the effects of PE-22-28 on post-stroke recovery used intraperitoneal injection as the route of administration . Researchers must determine optimal dosing based on literature and IACUC guidelines.
  • In vitro: Use low-protein-binding tubes and consider a carrier protein (e.g., 0.1% BSA) to prevent adsorption at low concentrations .
• Safety & Handling: Always use personal protective equipment (PPE) such as gloves and safety goggles when handling. Work in a laminar flow hood or well-ventilated area. Dispose of all waste in appropriate biohazard containers.

❓ Frequently Asked Questions (FAQ)

Q1: What is the difference between PE-22-28 and Spadin?

A: Spadin (PE 12-28) is a longer, naturally-derived peptide that also blocks the TREK-1 channel but has a short duration of action. PE-22-28 (Mini-Spadin) is a shorter, synthetic analog that was developed to have greater efficacy, higher potency, and improved in vivo bioavailability, making it a more robust tool for research .

Q2: What is the primary mechanism of action for PE-22-28?

A: Its primary mechanism is the potent and specific inhibition of the TREK-1 potassium channel (KCNK2). By blocking this channel, it modulates neuronal activity, leading to downstream effects like increased neurogenesis, synaptogenesis, and neuroprotection .

Q3: How do I verify the quality of your PE-22-28?

A: We are committed to transparency. Our product listings provide access to a third-party Certificate of Analysis (COA) . This document validates the compound's identity via Mass Spectrometry (MS) and its purity via High-Performance Liquid Chromatography (HPLC), which is ≥98% .

Q4: What are the primary research areas for PE-22-28?

A: It is primarily used in post-stroke recovery (ischemia) , depression and antidepressant research, neurogenesis and synaptic plasticity studies, and potentially in research on anxiety and stress-related disorders .

Q5: How should I store PE-22-28 after reconstitution?

A: Immediately prepare single-use aliquots. For long-term storage, store aliquots at -80°C for up to 6 months, or -20°C for up to 1 month. For short-term use (a few days), it can be stored at 2-8°C. Avoid repeated freeze-thaw cycles .

📦 Our Services & Guarantees

• 24/7 Customer Support: Our team is available to answer your pre- and post-sales questions.
• Authenticity Guarantee: We guarantee that you receive the exact product described, with full documentation and lot matching.
• Product Replacement: In the unlikely event of damage during shipping, we have a clear process for claims and replacement.
• Technical Consultation: Contact us for assistance with solubility or handling protocols.

🚀 How to Buy PE-22-28 Peptide Online

Purchasing from buypeptidesonlineusa.com is simple and secure.

1. Browse & Select: Choose the quantity you need (e.g., 1mg, 5mg vial).
2. Add to Cart: Proceed to our secure checkout.
3. Payment: We accept major credit cards and cryptocurrency (Bitcoin, Ethereum, USDT) for privacy and convenience.
4. Shipping: Orders are processed within 24 hours on business days and shipped in discreet, temperature-controlled packaging. Domestic orders typically arrive in 2-5 days.

Ready to advance your neuroprotection and depression research? Add high-purity PE-22-28 (Mini-Spadin) peptide to your cart today.