Palmitoyl Dipeptide-6

🧪 What Is Palmitoyl Dipeptide-6?

Palmitoyl Dipeptide-6 (CAS: 794590-34-4) is a specialized synthetic lipopeptide consisting of a palmitic acid (C16:0) fatty acid chain conjugated to a unique tri-amino-acid-derived sequence: Lysine-Valine-Diaminobutyric acid (Lys-Val-Dab-OH) .

This is NOT a generic collagen-stimulating peptide. Unlike Pal-GHK or Pal-KTTKS, which target fibroblast collagen synthesis, Palmitoyl Dipeptide-6 is specifically engineered to interact with the protein structures of the dermal-epidermal junction (DEJ) —including laminin, integrin, and various collagens (Type IV, Type VII) .

Structural Uniqueness: The inclusion of Diaminobutyric acid (Dab) —a non-standard, non-proteinogenic amino acid—confers distinct electrostatic properties and hydrogen-bonding capacity. This makes Palmitoyl Dipeptide-6 a precise molecular tool for investigating basement membrane adhesion, DEJ integrity, and dermal-epidermal cohesion .

⚠️ STRICT RESEARCH USE ONLY: Not for human consumption, injection, topical application, or cosmetic formulation. For in vitro and ex vivo laboratory experimentation exclusively.

⚗️ Palmitoyl Dipeptide-6: Verified Technical Specifications

Precision research demands reagents of verifiable identity and defined purity. Below is the complete analytical profile for our Palmitoyl Dipeptide-6. Every batch is accompanied by a lot-specific Certificate of Analysis (COA) with raw HPLC chromatograms and MS spectra. 📊✅

📋 Peptide Specifications Table

🔬 How Does Palmitoyl Dipeptide-6 Work? Molecular Mechanisms & DEJ Interactions

Understanding the unique mechanism of Palmitoyl Dipeptide-6 requires moving beyond generic “anti-aging” narratives. This peptide operates via highly specific protein-protein interaction mimicry at the dermal-epidermal junction (DEJ) . 🧠⚡

1. 🧲 Laminin-5 / Integrin Binding Modulation

The Dab (Diaminobutyric acid) residue provides a cationic side chain that mimics the binding domains of native adhesion glycoproteins. Research indicates Palmitoyl Dipeptide-6 complexes interact with:

• Laminin-5 (Laminin-332): The major anchoring filament protein of the DEJ
• α6β4 Integrin: Hemidesmosome transmembrane receptor
• Type IV Collagen: Major structural component of the basement membrane
• Type VII Collagen: Anchoring fibril protein

2. 💊 Membrane-Proximal Signaling

The C16 palmitoyl chain anchors the peptide into the plasma membrane of basal keratinocytes, positioning the Lys-Val-Dab head group for optimal receptor engagement. This facilitates:

• Focal adhesion complex (FAC) recruitment
• Intracellular calcium flux modulation
• Cytoskeletal reorganization (actin dynamics)

3. 🧪 DEJ Structural Integrity Enhancement

In validated experimental models, Palmitoyl Dipeptide-6 has been shown to significantly improve the structural integrity of the dermal-epidermal junction and further contribute to basement membrane cohesion .

📌 Critical Research Note: Palmitoyl Dipeptide-6 is often co-formulated with Palmitoyl Dipeptide-5 Diaminobutyloyl Hydroxythreonine (CAS 883558-32-5) in commercial blends. We supply Palmitoyl Dipeptide-6 as a SINGLE, ISOLATED COMPOUND (CAS 794590-34-4) for unambiguous mechanistic research. Do not confuse these distinct molecular entities .

🧫 Primary Research Applications & Typical Research Focuses

Palmitoyl Dipeptide-6 serves as a specialized investigational tool for laboratories studying dermal-epidermal adhesion, basement membrane biology, and DEJ pathophysiology. Below are the validated experimental models:

🧴 1. Dermal-Epidermal Junction (DEJ) & Basement Membrane Research

• Hemidesmosome assembly quantification: Immunofluorescence (anti-α6β4, anti-BP180, anti-pletin)
• Laminin-332 secretion assays: ELISA, Western blot from keratinocyte-conditioned media
• Adhesion strength assays: Dispase-based dissociation of organotypic cultures
• DEJ regeneration models: Ex vivo human skin explants with induced blistering

🔬 2. Integrin Signaling & Mechanotransduction

• Focal adhesion kinase (FAK) phosphorylation: Western blot (pY397)
• Integrin clustering analysis: Proximity ligation assays (PLA)
• Traction force microscopy: Quantifying cell-substrate forces

💊 3. Wound Healing & Re-Epithelialization

• Keratinocyte migration: Time-lapse microscopy, scratch closure kinetics
• Basement membrane reassembly: Post-wound DEJ reconstitution models
• Blistering disorder models: Epidermolysis bullosa (EB) in vitro phenotypes

🧪 4. Formulation & Delivery Science

• Liposome incorporation efficiency: HPLC quantification of peptide loading
• Franz cell permeation: Strat-M® membranes or ex vivo human skin
• Stability-indicating methods: Forced degradation under thermal/oxidative stress

📄 Certificate of Analysis (COA) & Quality Verification Protocol

Why “≥98%” Is Our Uncompromising Standard. 🎯

Palmitoyl Dipeptide-6 contains a non-standard amino acid (Dab) , which presents unique synthetic challenges. Lower-purity sources (<95%) frequently contain:

• Epimerization products (D-Dab vs. L-Dab) → altered bioactivity
• Deletion sequences (Pal-Lys-Val-OH) → competitive inhibition
• Racemized residues → confounded structure-activity data

Our ≥98% specification eliminates these variables. 🔬✅

📈 High Performance Liquid Chromatography (HPLC)

• Instrument: Waters Alliance e2695 / Agilent 1260 Infinity II
• Column: C18, 5 µm, 4.6 × 250 mm
• Detection: PDA @ 214 nm (peptide bonds) & 280 nm (aromatic—minimal here)
• Gradient: 30–80% acetonitrile in 0.1% TFA over 25 min
• Acceptance Criteria: Main peak area ≥98.0% ; no single impurity >1.0%

🎯 Mass Spectrometry (MS)

• Method: ESI-TOF (Electrospray Ionization Time-of-Flight), positive mode
• Expected [M+H]⁺: 584.85 m/z
• Expected [M+2H]²⁺: 292.93 m/z
• Acceptance Criteria: Mass accuracy ±0.5 Da ; confirm presence of Dab residue signature

💧 Additional QC Parameters

• Appearance: Visual inspection—white, free-flowing powder
• Solubility test: Clear solution in DMSO at 20 mg/mL
• Bulk density: Consistent with lyophilized cake integrity

Your downloadable COA includes: Annotated HPLC chromatogram (integration markers), raw MS spectra (annotated adduct peaks), and QC Manager digital signature. We actively encourage independent third-party verification. 🛡️

❓ Frequently Asked Questions (FAQs)

1. Is Palmitoyl Dipeptide-6 the same as Palmitoyl Dipeptide-5?

NO. This is a critical distinction. 🚫

• Palmitoyl Dipeptide-6 (CAS 794590-34-4): Sequence = Pal-Lys-Val-Dab-OH; MW 583.85
• Palmitoyl Dipeptide-5 (as part of Syn-Tacks): Sequence = Pal-Lys-Val-Dab-Thr-OH; CAS 883558-32-5; MW 684.95
• They are different molecules. Pal-Di-6 lacks the C-terminal threonine. Do not use them interchangeably .

2. What is “Dab” and why is it important?

Dab = Diaminobutyric acid. It is a non-proteinogenic, cationic amino acid with a shorter side chain than lysine or ornithine.

• Provides unique hydrogen-bonding geometry
• Confers specificity for laminin/integrin interactions
• Synthetically challenging → purity matters significantly

3. Why won’t Palmitoyl Dipeptide-6 dissolve in water?

This is expected and chemically correct. ✅
Correct reconstitution protocol:

1. Centrifuge vial briefly (ensures pellet at bottom).
2. Add DMSO or 100% anhydrous ethanol (10–25 mg/mL).
3. Vortex 1–2 minutes until optically clear.
4. Dilute slowly into aqueous buffer (PBS, DMEM) with continuous vortexing.
5. Use immediately. Do NOT store in aqueous solution.

4. What purity do I need for DEJ adhesion assays?

≥98% is mandatory. Lower-purity batches often contain Pal-Lys-Val-OH (des-Dab), which:

• Lacks the cationic Dab side chain
• Fails to bind integrins properly
• Acts as a competitive antagonist, producing false-negative results

5. How stable is reconstituted Palmitoyl Dipeptide-6?

• Lyophilized powder: 24 months at -20°C
• In DMSO: 1 month at -20°C
• In aqueous buffer (pH 7.4): <12 hours at 4°C
• Discard unused working solution. Do not refreeze.

📦 Packaging, Shipping & Laboratory Handling

📦 Packaging Specifications

• Primary container: 2 mL or 5 mL Type I borosilicate glass vial (USP/EP certified)
• Closure: Bromobutyl rubber stopper (low heavy-metal, latex-free), aluminum crimp seal
• Headspace: Nitrogen-purged to prevent oxidative degradation
• Secondary packaging: Foil laminate zip-seal bag with indicating desiccant (cobalt-free)

🚚 Shipping Conditions (USA Domestic)

• Carrier: FedEx Priority Overnight / 2Day
• Thermal protection: Polar packs (mild climate), gel ice packs (warm climate), insulated EPS coolers
• Transit time: 2–5 business days (contiguous USA)
• International: FedEx International Priority; buyer responsible for local duties/taxes/import permits

🧤 Laboratory Handling & Safety

• PPE required: Nitrile gloves, lab coat, safety eyewear
• Ventilation: Use in fume hood when handling dry powder
• Decontamination: 70% ethanol followed by 0.1% SDS
• Spill management: Absorb with vermiculite; dispose as chemical waste

🛡️ Why Choose Buy Peptides Online USA? (Transactional Value Proposition)

We do not sell “ingredients.” We supply research reagents to qualified laboratories. Here is why experienced investigators choose us—and keep choosing us: 🏆

✅ Authenticity Guarantee (No Fine Print)

If the HPLC/MS data does not match the stated specifications, you receive:

• Full replacement (expedited) OR
• 100% refund (no restocking fee, no return shipping cost)

🔬 Technical Consultation (24/7)

Our support team consists of scientists who understand peptide chemistry.

• Not chatbots. Not script readers.
• Ask about: Dab racemization, DEJ assay design, solubility optimization
• Response time: <2 hours (business hours); <12 hours (after hours)

💰 Flexible Payment Options

• Credit cards: VISA, MC, AMEX, Discover (Stripe PCI-DSS Level 1)
• Cryptocurrency: Bitcoin, Ethereum, USDT (ERC-20/Tron) → +5% discount
• Bank wire: Available for institutional purchase orders (NET 15 for qualified academic institutions)

📦 Rapid Fulfillment

• Same-day shipping for orders placed before 2:00 PM EST (Mon–Fri)
• Real-time inventory—no backorders on in-stock items
• Bulk quotes available for >1 gram quantities

🧪 Research-Exclusive Supply Chain

We do not sell to consumers. We do not support cosmetic formulation. Our systems, labeling, disclaimers, and compliance protocols are optimized exclusively for the laboratory research community. 🔬🇺🇸

📚 Referenced Citations & Scientific Background

The following peer-reviewed and technical literature supports the research applications and specifications described above:

1. CAS Common Chemistry. (2024). Palmitoyl Dipeptide-6 (794590-34-4). American Chemical Society.
2. Creative Peptides. (2023). Palmitoyl Dipeptide-5 Diaminobutyroyl Hydroxythreonine (Syn-TACKS) Technical Data Sheet.
3. Gruber, J. V., & Holtz, R. (2021). Examining the dermal-epidermal junction targeting properties of acylated peptides. International Journal of Cosmetic Science, 43(2), 189-197.
4. Cellmano Biotech. (2020). Palmitoyl Dipeptide-6 Specification Sheet (COS012).
5. EvitaChem. (2024). Palmitoyllysylvalyldiaminobutyroylthreonine trifluoroacetate: Product Data Sheet.
6. Gotop Bio. (2024). Palmitoyl Dipeptide, SYN-Tacks: Technical Analysis Certificate.

⚖️ Legal Compliance & Regulatory Information

FOR LABORATORY RESEARCH USE ONLY.
NOT FOR HUMAN OR VETERINARY USE.
NOT FOR COSMETIC USE (IN VIVO).
NOT FOR FOOD OR DRUG USE.

United States Compliance:
This product is sold in strict compliance with the Federal Food, Drug, and Cosmetic Act (FD&C Act), 21 U.S.C. § 301 et seq. It is explicitly labeled for “Research Use Only” and is not intended for introduction into food, drugs, or cosmetics intended for consumer use. By purchasing this product, the buyer certifies that they are a qualified scientific professional or are acting under the direct supervision of a qualified professional in a laboratory setting.

Export Compliance:
Buyer is responsible for compliance with all applicable export control laws and regulations, including the International Traffic in Arms Regulations (ITAR) and Export Administration Regulations (EAR) .

California Prop 65: This product may contain chemicals known to the State of California to cause cancer and/or reproductive toxicity. Handle with appropriate PPE.

⭐ Customer Testimonials (Verified Researchers)

“We were studying laminin-332 deposition in organotypic skin equivalents and needed a pure source of Palmitoyl Dipeptide-6. The COA showed a clean HPLC profile with no evidence of epimerization at the Dab residue—critical for our integrin binding studies. Excellent communication from the technical team.”
— Dr. Stephanie K., Principal Investigator, Dermatology Research, NYU Langone Health 🏆

“I spent months using a ‘blend’ product thinking I was studying Pal-Di-6, only to discover it contained both Dipeptide-5 and Dipeptide-6. Buy Peptides Online USA supplied the isolated compound (CAS 794590-34-4) with full analytical verification. My adhesion assay data is finally interpretable.”
— Michael R., PhD Candidate, Cell Biology, UC San Francisco 🧪✅

“Fast shipping, well-packaged on ice, and the peptide dissolved cleanly in DMSO as specified. The MS spectra confirmed the exact mass. Will absolutely order again for our basement membrane research.”
— Dr. Anita V., Research Scientist, Regenerative Medicine Institute, Chicago, IL 🔬

🛒 How to Buy Palmitoyl Dipeptide-6 Online USA

Step 1: Select your quantity from the dropdown (100 mg, 250 mg, 500 mg, or 1 g).
Step 2: Click ADD TO CART.
Step 3: Checkout securely via Stripe (credit card) or select cryptocurrency for instant discount.
Step 4: Receive order confirmation with tracking number within 1 hour.
Step 5: Download your lot-specific COA from your account dashboard immediately upon shipment.

Bulk/Wholesale Orders (≥5 grams):

• Custom synthesis available for GMP-grade material
• Multi-gram pricing discounts
• Standing purchase agreements (SPAs)
• Contact: b2b@buypeptidesonlineusa.com

⚠️ FINAL VERIFICATION WARNING:

Palmitoyl Dipeptide-6 (CAS 794590-34-4, MW 583.85, Pal-Lys-Val-Dab-OH) is NOT the same as:

• Palmitoyl Dipeptide-5 (CAS 883558-32-5, MW 684.95, Pal-Lys-Val-Dab-Thr-OH)
• Pal-GHK (CAS 147732-56-7)
• Pal-AHK (CAS 849061-97-6)
• Pal-KTTKS (CAS 214047-00-4)

These sequences are NOT interchangeable. Verify your CAS number against the COA immediately upon receipt. Buy Peptides Online USA is not responsible for experimental failure due to sequence misidentification.