IGF-1 LR3 (Receptor Grade)

🧪 Unleash Extended Signaling Research with IGF-1 LR3: The Engineered, Long-Acting Growth Factor for Demanding Cellular Assays

Welcome to the definitive source for Receptor-Grade IGF-1 LR3 Peptide, a scientifically engineered, long-acting analog of Insulin-like Growth Factor 1 designed for extended-duration cellular research and complex experimental models. 🚀 At Buy Peptides Online USA, we equip research laboratories with the highest-caliber, analytically verified signaling molecules. This comprehensive technical guide provides the exacting specifications, specialized handling protocols, and detailed scientific context required to confidently deploy IGF-1 LR3 in your studies on sustained mitogenic signaling, tissue hypertrophy, and cellular survival pathways. Our entire process is built upon a foundation of advanced analytical verification, recombinant purity, and an unwavering commitment to research-only integrity, delivering a predictable and potent reagent for critical investigations.

🛒 Secure your supply of this premier research growth factor. Click to add Receptor-Grade IGF-1 LR3 to your cart. Benefit from fast USA shipping and free delivery on orders over $300!

🔬 What is IGF-1 LR3 (Long R3 IGF-1)?

IGF-1 LR3 (Long R3 Insulin-like Growth Factor-I) is not a naturally occurring peptide but rather a synthetically engineered, 83-amino acid analog of human IGF-1. It incorporates two key modifications that make it a superior tool for many in-vitro research applications: 1) a 13-amino acid N-terminal extension peptide, and 2) a substitution of Arginine for Glutamic Acid at position 3 (R3).

These deliberate changes confer two major research advantages. First, the altered sequence dramatically reduces its binding affinity for all IGF Binding Proteins (IGFBPs), increasing its free, bioactive concentration in cell culture media. Second, the modifications result in a significantly extended functional half-life in experimental systems compared to native IGF-1 or the DES variant. This allows researchers to study sustained IGF-1 receptor (IGF-1R) activation over longer periods without frequent re-dosing, making it ideal for multi-day differentiation assays, chronic hypertrophy studies, and complex tissue modeling. 🧫

📊 IGF-1 LR3 (Receptor Grade): Complete Product Specifications

For research demanding the highest level of characterization, here is the complete analytical profile of our Receptor-Grade IGF-1 LR3.

⚙️ Primary Research Functions & Key Investigative Pathways

IGF-1 LR3 is a workhorse growth factor in laboratories focused on sustained anabolic signaling, tissue engineering, and developmental biology. Its engineered longevity makes it uniquely suited for chronic exposure experiments.

🔍 Typical Research Focuses Include:

• Sustained Myotube Hypertrophy & Differentiation: Long-term studies on C2C12 or primary myoblast cultures to assess cumulative effects on muscle protein synthesis, myotube diameter, and differentiation markers like Myogenin. 💪
• Neuronal Cell Culture & Protection: Extended-duration assays investigating trophic support for PC12 cells or primary neurons, examining neurite outgrowth and protection against chronic insult models. 🧠
• Chondrocyte & Osteoblast Culture: Research into the prolonged effects on cartilage and bone cell matrix production, proliferation, and phenotype maintenance in 3D culture systems. 🦴
• Stem Cell Proliferation & Lineage Commitment: Investigations into the role of sustained IGF-1R signaling in maintaining stemness or directing the differentiation of mesenchymal stem cells (MSCs).
• IGF-1 Receptor (IGF-1R) Signaling Pathway Analysis: A superior ligand for studies on receptor internalization kinetics, downstream kinase activation (AKT, MAPK), and cross-talk with insulin receptors over time.

⚠️ Critical Research Notice: The applications described are standard within the scientific community for this well-characterized research reagent. IGF-1 LR3 is sold exclusively as a research chemical for controlled laboratory investigations. This product is not for human or veterinary therapeutic use, is not a dietary supplement, and has not been evaluated by the FDA.

✅ Why Choose Our Receptor-Grade IGF-1 LR3 for Your Laboratory?

When you order IGF-1 LR3 online from our USA-based distribution hub, you are selecting a partner that prioritizes the success of your signaling research. Here is our unequivocal commitment:

• “Receptor-Grade” Analytical Stringency: Our “Receptor Grade” designation means each batch is validated not just for purity (≥99% via HPLC), but specifically for correct folding and bioactivity potential, confirmed by Mass Spectrometry (MS). It is ideal for sensitive receptor-binding and cellular response assays.
• Optimized for Complex, Long-Duration Assays: The engineered long half-life of IGF-1 LR3 provides more consistent stimulation in multi-day experiments, reducing media change frequency and improving data consistency in differentiation or hypertrophy studies.
• Uncompromising Research-Only Compliance: We serve only qualified researchers and institutions. Our documentation is precise, our marketing is factual, and we maintain strict adherence to the sale of materials for laboratory investigation only.
• Reliable Cold-Chain Domestic Fulfillment: We ship from within the USA using insulated packaging with ample cold packs or dry ice to ensure stability. Free shipping on all orders over $300.
• Expert Technical Support & Secure Procurement: Our team provides technical consultation on reconstitution and application. Purchase with confidence using major credit cards or our secure, private cryptocurrency payment option (Bitcoin, Ethereum, USDT).

👉 Click ‘Add to Cart’ to acquire your Receptor-Grade IGF-1 LR3. Equip your lab with a growth factor reagent of proven consistency and performance.

🧫 Advanced Handling, Reconstitution & Cell Culture Protocol

⚠️ ATTENTION: IGF-1 LR3 is a large, delicate recombinant protein. Improper handling will destroy its bioactivity. Follow this validated protocol meticulously.

1. Pre-Experiment Planning: Pre-chill all solvents and equipment on ice. Plan your final desired concentration and aliquot strategy before opening the vial.
2. Storage of Lyophilized Powder: Upon receipt, immediately transfer the unopened vial to a -80°C freezer for long-term storage. For daily use, a dedicated -20°C freezer is acceptable, but -80°C is strongly recommended for maximum shelf life.
3. Initial Reconstitution (The Most Critical Step): DO NOT USE water, PBS, or culture medium. ONLY use a sterile, weak acidic solution. 10mM HCl (pH ~2.0) or 0.1M acetic acid is standard. Gently pipette the cold acid down the inner wall of the vial. Let it sit undisturbed on ice for 30-60 minutes. After this, gently swirl or flick the vial. NEVER VORTEX. The solution should be clear. Cloudiness indicates aggregation and probable denaturation.
4. Creation of a Concentrated Stock Solution: This clear acid solution is your primary stock (e.g., 1 mg/mL). Immediately aliquot this primary stock into sterile, low-protein-binding microcentrifuge tubes (e.g., 10 µL per aliquot). Store all aliquots at -80°C. Each aliquot is for single use only.
5. Preparation of Working Medium: For your experiment, thaw one aliquot on ice and dilute it directly into your pre-warmed, pH-buffered cell culture medium to the final working concentration (typically 10-100 ng/mL). The small volume of acid will be neutralized by the medium’s buffer.

👨‍🔬 Essential Laboratory Safety & Best Practices:

• This product is for use by trained personnel in a designated cell culture laboratory.
• Wear full PPE: lab coat, gloves, and safety glasses. Use aseptic technique in a laminar flow hood for all steps involving open vials.
• Use only low-protein-binding, sterile filter tips and tubes to prevent peptide loss through adsorption.
• Decontaminate all waste and consumables in contact with the peptide according to your institution’s biological waste protocols.

🚫 WARNING: FOR RESEARCH USE ONLY. STRICTLY NOT FOR HUMAN OR VETERINARY USE. IGF-1 LR3 is a potent, engineered research-grade growth factor. It is intended solely for controlled in-vitro (cell culture) or ex-vivo experimental applications. It is not for injection, ingestion, or any form of human or animal administration. It is not a supplement or drug.

📦 Specialized Packaging, Shipping & Service Guarantee

• Guaranteed Cold-Chain Packaging: Your IGF-1 LR3 is sealed under inert gas, packed with desiccant, and shipped in a robust insulated container with sufficient frozen gel packs or dry ice to maintain a frozen state during transit, all inside a plain outer box.
• Rapid USA & Global Research Logistics: We offer priority USA shipping with full tracking. International orders for qualified institutions include all necessary customs documentation declaring the material as a “Research Chemical.”
• Our Service Commitment: Your investment is secured by our authenticity guarantee, validated by the batch-specific CoA. We provide 24/7 support for order inquiries and a clear product replacement policy for issues verified upon receipt.

❓ Frequently Asked Questions (FAQs)

Q1: What does “Receptor Grade” specifically mean for this product?

A1: Our “Receptor Grade” designation indicates the IGF-1 LR3 has undergone additional analytical scrutiny to ensure it is properly folded and suitable for studies directly involving the IGF-1 Receptor (IGF-1R). This includes verification via Mass Spectrometry (MS) that confirms the correct molecular weight and structure, making it reliable for binding assays, signaling studies, and other sensitive applications where bioactivity is paramount.

Q2: What is the difference between IGF-1 DES and IGF-1 LR3 for my research?

A2: The core difference is half-life and experimental design. IGF-1 DES is a short, potent burst ligand ideal for acute signaling studies (minutes to hours). IGF-1 LR3, with its binding protein resistance, is engineered for chronic, sustained exposure experiments over days. Choose LR3 for differentiation, hypertrophy, or any assay requiring prolonged receptor stimulation.

Q3: What is a typical working concentration for cell culture?

A3: As a supplier, we cannot prescribe experimental parameters. However, the published scientific literature commonly uses IGF-1 LR3 in a range of 10 to 100 ng/mL in cell culture media for various cell types. You must conduct a literature search for your specific cell line and perform a pilot dose-response experiment to determine the optimal concentration for your assay.

Q4: My reconstituted aliquot turned cloudy after thawing. Is it still usable?

A4: Cloudiness indicates protein aggregation and probable loss of bioactivity. It should not be used for quantitative experiments. This is often caused by improper initial reconstitution (using the wrong solvent), excessive agitation, or a failed freeze-thaw cycle. Always reconstitute in weak acid, aliquot immediately, avoid freeze-thaw cycles, and inspect each aliquot before use.

Q5: Do you offer bulk pricing for core facility or large-scale projects?

A5: Yes. We provide competitive bulk pricing for core facilities, academic laboratories, and commercial research teams requiring larger quantities. Please contact our sales team directly via the website for a custom quote.

📝 How to Order & Final Researcher Advisory

IGF-1 LR3 is an indispensable tool for modern cell biology research requiring sustained growth factor signaling. By selecting our Receptor-Grade product, you are investing in a reagent characterized for reliability in your most demanding, long-duration assays.

To place your order:

1. Select the required quantity of IGF-1 LR3 (Receptor Grade).
2. Click “Add to Cart” and proceed to review your order.
3. Continue to our secure checkout to finalize shipping and payment.
4. Review and confirm. You will receive an instant email confirmation with order details.

We are dedicated to enabling discovery. For bulk orders, institutional POs, or detailed technical consultations on assay integration, please contact our specialized support team.

Disclaimer: All information contained herein is for educational and descriptive purposes related to the research compound’s properties. It is not safety, procedural, or dosage advice. Purchasers must verify that their purchase and use comply with all applicable local, state, federal, and institutional guidelines and regulations. This product is for use by trained professionals in a controlled laboratory setting only.