GHRP-2 Peptide

🔬 Introduction: The High-Potency GHSR-1a Agonist

Welcome to Buy Peptides Online USA, the trusted source for high-purity research peptides online. Our GHRP-2 Peptide (Pralmorelin) is a synthetic hexapeptide and one of the most potent Growth Hormone Secretagogue Receptor (GHSR-1a) agonists available for laboratory research.

Formerly known as KP-102 or Pralmorelin (INN) , GHRP-2 is distinguished from first-generation GHRPs (GHRP-6) and selective analogs (Ipamorelin) by its superior GH secretory amplitude and high receptor binding affinity .

Why choose GHRP-2?

• Maximal GH Pulse Amplitude: Consistently demonstrates higher GH release than GHRP-6 or Ipamorelin in standardized pituitary assays .
• Dual Research Utility: The gold standard for modeling both somatotroph activation and ghrelin-mediated appetite pathways .
• Enzymatic Stability: D-amino acid substitutions (D-Ala¹, D-Phe⁵, D-β-Nal²) confer resistance to plasma degradation .
• Clinical Benchmark: Pralmorelin is an approved diagnostic agent for GH deficiency in Japan (GHRP-2 stimulation test) .

This product is strictly for in vitro and laboratory research use only.

✅ Why Choose This GHRP-2 Peptide from Buy Peptides Online USA?

The peptide research market is saturated with undefined “research liquids” of questionable provenance. We differentiate through analytical transparency and sequence fidelity.

Why does this matter? In GHSR-1a binding studies, impurities and incorrect diastereomers (e.g., L-Phe instead of D-Phe) render the peptide inactive. Our ≥99% purity and ESI-MS verification guarantee full biological potency.

🧪 What Is GHRP-2 (Pralmorelin)? (Mechanistic Deep-Dive)

🔬 Definition & Nomenclature

GHRP-2 (Growth Hormone Releasing Peptide-2) is a synthetic hexapeptide belonging to the family of Growth Hormone Secretagogues (GHSs) . It is a potent, full agonist at the GHSR-1a receptor (ghrelin receptor) .

International Nonproprietary Name (INN): Pralmorelin
Development Code: KP-102
Regulatory Status: Approved in Japan as a diagnostic agent for GH deficiency (GHRP-2 stimulation test) .

🧬 Peptide Structure & Sequence

GHRP-2 is engineered with strategic D-amino acid substitutions to maximize metabolic stability and receptor affinity .

Critical Structural Features:

1. D-Ala¹: Protease resistance (aminopeptidase).
2. D-β-Nal²: Naphthylalanine; significantly increases hydrophobic binding to GHSR-1a transmembrane domains.
3. D-Phe⁵: Confers resistance to carboxypeptidases.
4. C-Terminal Amidation (-NH₂): Essential for full agonist potency .

⚙️ How Does GHRP-2 Work? Signal Transduction Cascade

GHRP-2 is a full agonist at the Growth Hormone Secretagogue Receptor type 1a (GHSR-1a) , a G-protein-coupled receptor (GPCR) highly expressed in the pituitary, hypothalamus, and vagal nerve endings .

Step-by-Step Signaling Pathway :

1. Ligand Binding: GHRP-2 binds to the transmembrane heptahelical core of GHSR-1a.
2. Gαq/11 Activation: Unlike GHRH (Gαs), GHSR-1a primarily couples to Gαq/11 .
3. PLC Activation: Phospholipase C hydrolyzes PIP₂ → IP₃ + DAG.
4. Calcium Mobilization: IP₃ binds to receptors on the endoplasmic reticulum, releasing intracellular Ca²⁺.
5. PKC Activation: DAG activates Protein Kinase C.
6. GH Exocytosis: Elevated Ca²⁺ triggers vesicle fusion and GH release.
7. Somatostatin Antagonism: GHSR-1a activation in the hypothalamus suppresses somatostatin secretion, disinhibiting pituitary GH release .

GHRP-2 vs. Other GHRPs :
| Parameter | GHRP-2 | GHRP-6 | Ipamorelin | Hexarelin |
| :— | :— | :— | :— | :— |
| Relative GH Potency | High | Moderate | Moderate | Very High |
| ACTH/Prolactin Release | Moderate | Moderate | Negligible | Moderate |
| Receptor Binding Affinity | Very High | Moderate | High | Very High |
| Appetite Stimulation | Strong (GHSR-1a) | Strong | Weak | Strong |

📊 Peptide Specifications & Technical Data

Verified via Third-Party RP-HPLC and ESI-MS. COA available upon request.

🔬 Primary Research Applications & Investigational Focus

This product is for laboratory research only. The following outlines mechanisms reported in peer-reviewed, non-human studies .

📈 1. GHSR-1a Pharmacology & Signal Bias

GHRP-2 is the reference agonist for studying GHSR-1a constitutive activity and ligand bias . Researchers utilize GHRP-2 to:

• Quantify β-arrestin recruitment vs. Gq/11 signaling .
• Investigate receptor dimerization (GHSR-1a: melanocortin-3 receptor crosstalk) .
• Model GHSR-1a inverse agonism (e.g., using [D-Arg¹,D-Phe⁵,D-Trp⁷,9,Leu¹¹]-substance P) .

🧠 2. Appetite & Energy Homeostasis (Ghrelin Axis)

GHRP-2 is a powerful tool for studying the orexigenic (appetite-stimulating) arm of the ghrelin system. It is utilized in:

• Hypothalamic ARC neuron activation (NPY/AgRP neuron firing assays).
• Vagal afferent signaling models.
• Conditioned place preference (food reward/reinforcement) studies .

💪 3. Maximal GH Secretion & Somatotroph Reserve

The GHRP-2 stimulation test is a clinical diagnostic standard. In research, GHRP-2 is used to:

• Assess pituitary somatotroph reserve in rodent models of hypopituitarism .
• Study GH-IGF-1 axis negative feedback resistance .
• Model the synergistic GH release of GHRH + GHRP (co-administration studies) .

🩺 4. Gastrointestinal Motility & Gastric Emptying

As a ghrelin mimetic, GHRP-2 accelerates gastric emptying and small intestinal transit. It is employed in:

• Diabetic gastroparesis models.
• Post-operative ileus research .
• Enteric nervous system (ENS) neurochemistry studies .

🔬 5. ACTH & Cortisol Axis Interaction

Unlike Ipamorelin, GHRP-2 stimulates significant ACTH and cortisol release in preclinical models . This makes GHRP-2 valuable for researchers investigating:

• HPA axis crosstalk with the somatotropic axis.
• Stress-induced GH suppression mechanisms .
• Pituitary corticotroph-somatotroph paracrine signaling .

🧪 Quality Verification: HPLC, Mass Spectrometry & COA

We do not rely on spot-checking. Every batch of our GHRP-2 Peptide undergoes rigorous analytical chemistry validation per USP/EP guidelines .

1. Reverse-Phase High Performance Liquid Chromatography (RP-HPLC):
   • Method: C18 Column, Gradient Elution (0.1% TFA/ACN).
   • Criteria: Purity ≥99.0% . Single principal peak with retention time matching certified Pralmorelin reference standard .
   • Scrutiny: Absence of des-Lys⁶ (major impurity), D-amino acid epimerization, and oxidation products (Trp⁴).
2. Mass Spectrometry (MS):
   • Method: Electrospray Ionization (ESI-MS).
   • Criteria: 817.97 ± 1.0 Da. Confirms full-length, correctly amidated hexapeptide.
3. Endotoxin Analysis (LAL Test):
   • Method: Kinetic Chromogenic LAL.
   • Criteria: <1.0 EU/μg. Suitable for sensitive primary pituitary and hypothalamic cell cultures.
4. Certificate of Analysis (COA):
   A batch-specific COA is available immediately upon request. This document includes:
   • Lot Number & Manufacturing Date
   • Retention Time & Purity Percentage (HPLC Chromatogram)
   • MS Spectrum & Molecular Weight Confirmation
   • Appearance & Solubility Verification

❄️ Handling, Storage & Reconstitution Protocol

For Laboratory Use Only – Not for Human Consumption.

📦 Long-Term Storage (Lyophilized)

• Temperature: Store at -20°C (-4°F) in a manual-defrost freezer .
• Stability: Lyophilized powder is stable for 24 months at -20°C. Stable at room temperature for up to 4 weeks .
• Environment: Keep desiccated. Protect from UV light and humidity.

💧 Reconstitution Instructions (Standard Laboratory Protocol)

Based on standard research protocols for GHRPs :

1. Equilibrate: Allow the vial to reach room temperature in a desiccator to prevent moisture condensation.
2. Inject: Using a sterile syringe, inject 1.0 mL of sterile 18 MΩ·cm H₂O, Bacteriostatic Water (BAC), or PBS slowly against the inner glass wall.
   • Resulting Concentration: 5.0 mg/mL .
3. Swirl: Do NOT vortex or shake vigorously. Shaking introduces oxygen radicals and shears peptide bonds. Gently swirl until the liquid is clear.
4. Solubility Note: GHRP-2 is highly soluble (>20 mg/mL). No acidification required.

🧊 Post-Reconstitution Handling

• Storage: 2-8°C (Refrigerator). Do not freeze .
• Duration: Use within 30 days. GHRP-2 is stable in solution at 2-8°C for several weeks.
• ⚠️ Critical Alert: Freezing reconstituted peptides causes ice crystal shearing, permanently destroying the tertiary structure. Do not refreeze.
• Stabilization: For long-term storage (>30 days), aliquot and store at -80°C. Avoid freeze-thaw cycles.

📦 Packaging, Shipping & Buyer Services

We engineer the unboxing experience to match the precision of our analytics.

• 📐 Discreet & Secure Packaging: Vials are seated in medical-grade foam inside rigid, opaque mailers.
• 🌡️ Thermal Integrity: Seasonal cold-pack inserts included to maintain ≤4°C during summer transit.
• 🇺🇸 USA Dispatch: Shipped from domestic logistics hubs. Transit time: 2-5 business days.
• 🛡️ Buyer Guarantees:
  • Authenticity Guarantee: Direct sourcing from cGMP-compliant, FDA-registered facilities.
  • 24/7 Technical Consultation: Lab handling queries answered via email support.
  • Product Replacement: Immediate replacement for damaged or compromised vials (photographic proof required).
  • Complaint Handling: Dedicated resolution team for shipping discrepancies.

❓ Frequently Asked Questions (FAQ)

Q: What is the difference between GHRP-2 and GHRP-6?

A: Potency and Structure. GHRP-2 (Pralmorelin) is a hexapeptide with D-β-Nal² and D-Phe⁵ substitutions. GHRP-6 is a hexapeptide with L-Trp⁴ and D-Phe⁵. GHRP-2 is significantly more potent (2-3x) than GHRP-6 in stimulating GH release and has higher GHSR-1a binding affinity .

Q: What is the purity of this peptide?

A: ≥99.0% . This is verified via RP-HPLC (area normalization) and ESI-MS for each batch. We do not sell “≥95%” or unverified material. Batch-specific COAs are available immediately upon request.

Q: Does GHRP-2 stimulate cortisol and prolactin?

A: Yes, in most preclinical models. Unlike Ipamorelin, GHRP-2 reliably stimulates ACTH, cortisol, and prolactin secretion. This is a feature, not a bug, for researchers studying HPA-GH axis crosstalk or stress-induced GH modulation . For studies requiring GH selectivity, Ipamorelin is the preferred tool .

Q: Is GHRP-2 the same as Pralmorelin?

A: Yes. Pralmorelin is the International Nonproprietary Name (INN) for GHRP-2 (KP-102) . The terms are synonymous.

Q: What is the half-life of GHRP-2?

A: The plasma half-life in preclinical models is approximately 30-60 minutes . The D-amino acid content provides significant resistance to enzymatic degradation compared to non-substituted peptides.

Q: Is this peptide suitable for in vivo animal research?

A: Our products are labeled “For Research Use Only. Not for Human Consumption.” Determining suitability for specific IACUC-approved animal protocols is the responsibility of the qualified researcher and their institutional review board.

Q: Do you provide dosing instructions?

A: Absolutely not. As a strict Research Use Only (RUO) supplier, we strictly prohibit providing protocols for human or animal administration. We supply the raw material; determining molar concentrations for specific in vitro assays (e.g., 10nM vs. 100nM) is the responsibility of the qualified researcher.

⚖️ Legal & Compliance Disclaimer

Research Use Only (RUO)
This product is sold exclusively for laboratory research and educational purposes. It is NOT a drug, food, dietary supplement, or cosmetic. This product has not been approved by the U.S. Food and Drug Administration (FDA) .

WADA Prohibited Substance:
Growth Hormone Secretagogues (GHSs), including GHRP-2 (Pralmorelin), are explicitly included on the World Anti-Doping Agency (WADA) Prohibited List at all times (both in- and out-of-competition) . Bodily introduction of any kind, including human or veterinary consumption, injection, or topical application, is strictly prohibited and illegal .

By purchasing from Buy Peptides Online USA, you certify that you are a qualified researcher, scientist, or laboratory professional and will handle this material in compliance with all applicable local, state, and federal regulations, including institutional biosafety protocols.

⭐ Transactional Close: Why Principal Investigators Buy Here

Ready to investigate the ghrelinergic control of GH and appetite with the reference standard agonist? Here is why top neuroendocrinology and metabolic labs buy peptides online from Buy Peptides Online USA:

• 💰 Verified Value: 5mg/vial of sequence-verified, ≥99% pure GHRP-2 (Pralmorelin) at competitive pricing.
• ⚡ Velocity: Same-day processing on orders placed before 2:00 PM EST.
• 🔒 Secure Checkout: Accepting major credit cards and cryptocurrency (BTC/ETH/USDT) for institutional privacy and discretion.
• 📞 Post-Sale Support: Direct access for technical consultation regarding solubility, handling, or analytical data interpretation.

📄 Request a COA: Use our contact form with your Order Number or Batch Lot Number to receive the full analytical package, including HPLC chromatograms and MS spectra.