Fragment, Modified GRF, Ipamorelin (Blend)

🔬 Introduction: The Pulsatile Lipolytic Triad

Welcome to Buy Peptides Online USA, the trusted source for high-purity research peptides online. Our Fragment, Modified GRF, Ipamorelin Blend is a precision-formulated research tool designed for laboratories investigating the intersection of adipocyte metabolism and somatotroph dynamics.

This blend unites three mechanistically distinct peptide families into a single, stable lyophilized vial :

1. HGH Fragment 176-191: A 17-amino acid C-terminal fragment of human Growth Hormone. Exerts selective lipolytic activity via β3-adrenergic receptor modulation, completely isolated from IGF-1-mediated mitogenic signaling .
2. Modified GRF 1-29 (CJC-1295 no DAC): A tetrasubstituted GHRH analog. The "Gold Standard" for modeling physiological GH pulsatility due to its DPP-4 resistant structure and ~30-minute half-life .
3. Ipamorelin: A highly selective GHSR-1a agonist (GHRP). Amplifies the GH pulse initiated by Modified GRF while demonstrating negligible effects on prolactin, ACTH, or cortisol in preclinical models .

Why this specific triad? This is the only commercially available blend that allows researchers to investigate three distinct axes simultaneously :

• Adipose tissue lipolysis & fatty acid oxidation (HGH Fragment)
• Pituitary GH synthesis & pulse amplitude (Modified GRF)
• Somatostatin antagonism & receptor cross-talk (Ipamorelin)

This product is strictly for in vitro and laboratory research use only.

✅ Why Choose This Triple Blend from Buy Peptides Online USA?

The peptide research market is saturated with undefined "research liquids" and binary blends. We differentiate through forensic transparency and triple-pathway precision.

Why does this matter? In metabolic and endocrine research, impurities and incorrect molar ratios introduce confounding variables that invalidate lipolysis assays, cAMP quantification, and GH ELISA results. Our blend ensures your data reflects the biology, not the synthesis artifacts.

🧪 What Is the Fragment, Modified GRF & Ipamorelin Blend? (Mechanistic Deep-Dive)

🔹 Component A: HGH Fragment 176-191 – The Selective Lipolytic Agent

What is it?
HGH Fragment 176-191 is a synthetic 17-amino acid peptide derived from the C-terminal loop of the human Growth Hormone molecule (amino acids 176–191) .

Peptide Structure & Sequence:

• Sequence: Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe
• Molecular Formula: C₇₈H₁₂₁N₂₃O₂₂S₂
• Molecular Weight: ~1768.0 g/mol
• CAS Number: 126752-37-8 (Research Grade)
• Synonyms: hGH 176-191, Lipolytic Fragment, Growth Hormone Fragment

How Does It Work?
Unlike full-length rhGH, Fragment 176-191 does not bind the GH receptor dimer and therefore does not stimulate IGF-1 production or mitogenic signaling . Its mechanism is confined to adipocyte metabolism:

1. β3-Adrenergic Receptor Activation: Direct stimulation of adipocyte β3-ARs .
2. HSL Phosphorylation: Hormone-Sensitive Lipase is activated.
3. Triglyceride Hydrolysis: Adipose triglyceride is broken down into free fatty acids and glycerol.
4. Mitochondrial β-Oxidation: FAs are directed toward oxidation rather than re-esterification .

Research Advantage: This fragment allows researchers to model fat mass reduction and energy expenditure in isolation, without confounding anabolic/mitogenic signals .

🔹 Component B: Modified GRF 1-29 (CJC-1295 no DAC) – The GHRH Analog

What is it?
Modified GRF 1-29 is the correct scientific nomenclature for CJC-1295 without DAC. It is a tetrasubstituted, 29-amino acid synthetic analog of Growth Hormone-Releasing Hormone (GHRH) .

Peptide Structure & Sequence:

• Sequence: Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH₂
• Molecular Formula: C₁₅₂H₂₅₂N₄₄O₄₂
• Molecular Weight: ~3367.9 g/mol
• CAS Number: 863288-34-0 (Primary) / 446036-97-1 (Alternate)
• Synonyms: Mod GRF 1-29, CJC-1295 no DAC, GHRH(1-29)-NH₂, Tetrasubstituted GRF

How Does It Work?
Modified GRF 1-29 is a full agonist at the GHRH receptor on pituitary somatotrophs .

Signal Transduction Cascade:

1. Ligand Binding: Binds to GHRHR extracellular domain.
2. Gαs Activation: Conformational shift activates adenylyl cyclase.
3. cAMP Elevation: Intracellular cAMP concentration rises.
4. PKA Activation: Protein Kinase A phosphorylates CREB.
5. Gene Transcription: CREB binds to the GH1 gene promoter, initiating GH mRNA synthesis and vesicle exocytosis .

Pharmacokinetic Rationale (No DAC): The absence of the Drug Affinity Complex prevents albumin binding. This results in rapid clearance (t½ ≈ 30 min), allowing researchers to model discrete, pulsatile GH bursts rather than flat, continuous elevation .

🔹 Component C: Ipamorelin – The Selective GHRP

What is it?
Ipamorelin is a synthetic pentapeptide and a highly selective agonist at the GHSR-1a receptor (ghrelin receptor) .

Peptide Structure & Sequence:

• Sequence: Aib-His-D-2-Nal-D-Phe-Lys-NH₂
• Molecular Formula: C₃₈H₄₉N₉O₅
• Molecular Weight: ~711.9 g/mol
• CAS Number: 170851-70-4
• Synonyms: Selective Ghrelin Mimetic, GHRP-1 Analog

How Does It Work?
Ipamorelin mimics ghrelin's action, binding to GHSR-1a on somatotrophs and hypothalamic neurons. This suppresses somatostatin tone and amplifies the GH pulse initiated by GHRH .

Key Differentiator – Selectivity:
Unlike GHRP-2 or GHRP-6, Ipamorelin demonstrates exceptional specificity for GH release, with negligible effects on ACTH, prolactin, or cortisol secretion in preclinical models . This makes it the preferred GHRP for researchers seeking to isolate GH-specific signaling cascades without confounding stress hormone activation.

📊 Peptide Specifications & Technical Data

Verified via Third-Party HPLC-MS. COA available upon request.

🔬 Primary Research Applications & Investigational Focus

This product is for laboratory research only. The following outlines mechanisms reported in peer-reviewed, non-human studies .

🔥 1. Adipose Tissue Lipolysis & Mitochondrial Oxidation

The signature application of this triad. HGH Fragment 176-191 allows researchers to model β3-adrenergic lipolysis in primary adipocyte cultures or 3T3-L1 cell lines . Studies suggest the peptide may impact lipid mobilization independently of classic GH receptor pathways, making it a valuable tool for investigating non-canonical metabolic signaling .

📈 2. Pulsatile GH Secretion & Somatotroph Sensitivity

Modified GRF 1-29 provides the GHRH signal with physiological pulsatility, while Ipamorelin suppresses somatostatin. This blend is optimal for dose-response cAMP assays, GH release ELISA time-course studies, and investigations of somatotroph desensitization kinetics .

🧬 3. GHSR-1a Signal Bias & Receptor Cross-Talk

Ipamorelin is a biased agonist at the ghrelin receptor. This blend allows researchers to parse β-arrestin recruitment vs. Gq/11 signaling in the presence of concurrent GHRH stimulation. This is critical for understanding receptor desensitization and ligand-biased trafficking .

🧴 4. Cartilage & Connective Tissue Matrix Modeling

Emerging research suggests HGH Fragment 176-191 (via its AOD-9604 overlap) may influence cartilage regeneration and matrix metalloproteinase (MMP) regulation. A 2018 study utilizing the Fragment peptide demonstrated enhanced cartilage regeneration in osteoarthritic rabbit models .

💤 5. Sleep Architecture & Circadian Rhythms

GH secretion is tightly coupled to slow-wave sleep (SWS) . GHRH is intrinsically linked to NREMS regulation. This blend is employed in circadian rhythm research to model how augmented GH pulses influence sleep architecture, neural repair, and HPA axis regulation .

🧪 Quality Verification: HPLC, Mass Spectrometry & COA

We do not rely on spot-checking. Every batch of our Fragment, Modified GRF, Ipamorelin Blend undergoes rigorous analytical chemistry validation .

1. High Performance Liquid Chromatography (HPLC):

   • Method: Reverse-Phase C18 Column, Gradient Elution.
   • Criteria: Purity ≥99% per component.
   • Scrutiny: Absence of deletion sequences (incomplete synthesis) and oxidation products .

2. Mass Spectrometry (MS):

   • Method: Electrospray Ionization (ESI-MS).
   • Criteria: Confirmed molecular weights: 1768.0 Da, 3367.9 Da, 711.9 Da. This confirms full-length, correctly folded peptide chains .

3. Endotoxin Analysis (LAL Test):

   • Method: Kinetic Chromogenic LAL.
   • Criteria: <0.05 EU/mg. Significantly exceeds industry standard (≤5 EU/mg), ensuring compatibility with sensitive primary adipocyte and pituitary cell cultures .

4. Certificate of Analysis (COA):
   A batch-specific COA is available immediately upon request. This document includes:

   • Lot Number & Manufacturing Date
   • Retention Time & Purity Percentage
   • MS Spectrum & Molecular Weight Confirmation
   • Endotoxin Value & Appearance Verification

❄️ Handling, Storage & Reconstitution Protocol

For Laboratory Use Only – Not for Human Consumption.

📦 Long-Term Storage (Lyophilized)

• Temperature: Store at -20°C (-4°F) in a manual-defrost freezer .
• Stability: Lyophilized powder is stable for 12 months at -20°C. Stable at room temperature for up to 90 days, but refrigeration is recommended for archival storage .
• Environment: Keep desiccated. Protect from UV light and humidity.

💧 Reconstitution Instructions (Standard Laboratory Protocol)

Based on standard research protocols for triple GHRH/GHRP/Fragment blends :

1. Equilibrate: Allow the vial to reach room temperature in a desiccator to prevent moisture condensation.
2. Inject: Using a sterile syringe, inject 3.0 mL of Bacteriostatic Water (BAC) slowly against the inner glass wall.
   • Resulting Concentration: 5 mg/mL total peptide (1.67 mg/mL each of Fragment, Mod GRF, and Ipamorelin).
3. Swirl: Do NOT vortex or shake vigorously. Shaking introduces oxygen radicals and shears the peptide bonds. Gently swirl until the liquid is clear.

🧊 Post-Reconstitution Handling

• Storage: 2-8°C (Refrigerator). Do not freeze .
• Duration: Use within 21-28 days. Discard if cloudiness or particulate matter persists.
• ⚠️ Critical Alert: Freezing reconstituted peptides causes ice crystal shearing, permanently destroying the tertiary structure. Do not refreeze .

📦 Packaging, Shipping & Buyer Services

We engineer the unboxing experience to match the precision of our analytics.

• 📐 Discreet & Secure Packaging: Vials are seated in medical-grade foam inside rigid, opaque mailers.
• 🌡️ Thermal Integrity: Seasonal cold-pack inserts included to maintain ≤4°C during summer transit.
• 🇺🇸 USA Dispatch: Shipped from domestic logistics hubs. Transit time: 2-5 business days.
• 🛡️ Buyer Guarantees:
  • Authenticity Guarantee: Direct sourcing from cGMP-compliant, FDA-registered facilities.
  • 24/7 Technical Consultation: Lab handling queries answered via email support.
  • Product Replacement: Immediate replacement for damaged or compromised vials (photographic proof required).
  • Complaint Handling: Dedicated resolution team for shipping discrepancies.

❓ Frequently Asked Questions (FAQ)

Q: What exactly is "Modified GRF"? Is it the same as CJC-1295 no DAC?

A: Yes, they are synonymous. Modified GRF 1-29 is the scientific name for CJC-1295 without the Drug Affinity Complex (DAC) . It is a tetrasubstituted GHRH analog engineered for DPP-4 resistance and a ~30-minute half-life. It is not the same as Sermorelin (half-life <10 min) or CJC-1295 with DAC (half-life ~8 days) .

Q: What is the difference between Fragment 176-191 and AOD-9604?

A: Fragment 176-191 is 17 amino acids (176-191). AOD-9604 is 15 amino acids (177-191). Fragment 176-191 is the slightly longer, more stable variant widely preferred in lipolysis research due to its enhanced receptor affinity and enzymatic stability .

Q: What is the purity of this blend?

A: ≥99% per component. This is verified via HPLC (area normalization) and ESI-MS for each batch. We do not sell "≥98%" material .

Q: Why is Modified GRF (CJC no DAC) superior to Sermorelin in this blend?

A: Half-life. Sermorelin (GRF 1-29) is degraded by DPP-4 in <10 minutes. Modified GRF 1-29 has four strategic amino acid substitutions (D-Ala², Gln⁸, Ala¹⁵, Leu²⁷) that confer DPP-4 resistance, extending the half-life to ~30 minutes. This allows for a complete GH pulse to be modeled .

Q: Does HGH Fragment 176-191 stimulate IGF-1?

A: No. This is the critical distinction. Unlike full-length HGH, Fragment 176-191 does not bind the GH receptor dimer and therefore does not stimulate hepatic IGF-1 production or mitogenic signaling. Its effects are confined to adipocyte metabolism .

Q: How long is the shelf life after reconstitution?

A: When reconstituted with Bacteriostatic Water (0.9% benzyl alcohol), the solution is stable for 21-28 days at 2-8°C. Sterile water without preservatives shortens this window significantly .

Q: Do you provide dosing instructions?

A: Absolutely not. As a Research Use Only (RUO) supplier, we strictly prohibit providing protocols for human or animal administration. We supply the raw material; determining molar concentrations for specific in vitro assays (e.g., 10nM vs. 100nM) is the responsibility of the qualified researcher .

⚖️ Legal & Compliance Disclaimer

Research Use Only (RUO)
This product is sold exclusively for laboratory research and educational purposes. It is NOT a drug, food, dietary supplement, or cosmetic. This product has not been approved by the U.S. Food and Drug Administration (FDA) . Bodily introduction of any kind, including human or veterinary consumption, injection, or topical application, is strictly prohibited and illegal.

By purchasing from Buy Peptides Online USA, you certify that you are a qualified researcher, scientist, or laboratory professional and will handle this material in compliance with all applicable local, state, and federal regulations, including institutional biosafety protocols .

⭐ Transactional Close: Why Principal Investigators Buy Here

Ready to eliminate confounding variables from your metabolic and somatotroph signaling assays? Here is why top labs buy peptides online from Buy Peptides Online USA:

• 💰 Superior Value: 15mg total peptide mass (5mg Frag + 5mg Mod GRF + 5mg Ipa) at a competitive price point versus buying three individual vials.
• ⚡ Velocity: Same-day processing on orders placed before 2:00 PM EST.
• 🔒 Secure Checkout: Accepting major credit cards and cryptocurrency (BTC/ETH/USDT) for institutional privacy and discretion.
• 📞 Post-Sale Support: Direct access for technical consultation regarding solubility, storage stability, or analytical data interpretation.

📄 Request a COA: Use our contact form with your Order Number or Batch Lot Number to receive the full analytical package, including HPLC chromatograms and MS spectra.