CJC-1295 no DAC (Mod GRF 1-29)

🔬 Introduction: The Second-Generation GHRH Analog Standard

Welcome to Buy Peptides Online USA, the trusted source for high-purity research peptides online. Our CJC-1295 no DAC (Mod GRF 1-29) represents the gold standard in synthetic GHRH analog research.

This is not the long-acting CJC-1295 with Drug Affinity Complex (DAC). This is Modified GRF 1-29—a precise, tetrasubstituted 29-amino acid peptide engineered for enhanced enzymatic stability while preserving physiological pulsatility .

Why this distinction matters:

• CJC-1295 with DAC: Half-life ~8 days. Continuous receptor occupancy. Models sustained elevation.
• CJC-1295 no DAC (Mod GRF 1-29): Half-life ~30 minutes. Pulsatile clearance. Models endogenous GHRH bursts .

For researchers investigating somatotroph desensitization, negative feedback kinetics, or pulse-amplitude coding, the no-DAC variant is the biologically relevant tool .

This product is strictly for in vitro and laboratory research use only.

✅ Why Choose This CJC-1295 no DAC from Buy Peptides Online USA?

The peptide research market is saturated with undefined "research liquids" and mislabeled analogs. We differentiate through forensic transparency and sequence fidelity.

Why does this matter? In GHRH receptor binding assays, impurities and truncated sequences generate false positives/negatives. Our ≥99% purity ensures your cAMP/PKA/CREB data reflects receptor pharmacology, not synthesis artifacts .

🧪 What Is CJC-1295 no DAC (Mod GRF 1-29)? (Mechanistic Deep-Dive)

🔬 Definition & Nomenclature Clarification

CJC-1295 without DAC is properly termed Modified GRF (1-29) . It is a synthetic, tetrasubstituted analog of the endogenous 44-amino acid Growth Hormone-Releasing Hormone (GHRH) .

The Developmental Lineage:

1. GHRH (1-44): Endogenous hypothalamic hormone.
2. GRF (1-29) / Sermorelin: Bioactive core (first 29 amino acids). Half-life: <10 minutes (rapid DPP-4 degradation) .
3. Mod GRF 1-29 (CJC-1295 no DAC): Four strategic substitutions conferring DPP-4 resistance. Half-life: ~30 minutes .

Strategic Amino Acid Modifications:

• Position 2: L-Ala → D-Ala (Protease resistance)
• Position 8: Asn → Gln
• Position 15: Gly → Ala
• Position 27: Met → Leu (Oxidation prevention)
• C-Terminus: Free acid → NH₂ (Amidation)

🔬 Molecular Identity

🔬 Mechanism of Action: How Does Mod GRF 1-29 Work?

CJC-1295 no DAC is a full agonist at the GHRH receptor (GHRHR), a G-protein-coupled receptor (GPCR) located on pituitary somatotroph cells .

Signal Transduction Cascade:

1. Ligand Binding: Mod GRF 1-29 binds to the extracellular domain of GHRHR.
2. Gαs Activation: Conformational shift activates adenylyl cyclase.
3. cAMP Elevation: Intracellular cAMP concentration rises rapidly.
4. PKA Activation: Protein Kinase A phosphorylates CREB (cAMP Response Element-Binding protein).
5. Gene Transcription: CREB binds to the GH1 gene promoter, initiating GH mRNA synthesis.
6. Exocytosis: Pre-synthesized Growth Hormone vesicles are released into systemic circulation .

Pharmacokinetic Rationale (No DAC): The absence of the Drug Affinity Complex (DAC) moiety prevents albumin binding. This results in rapid clearance (t½ ≈ 30 min), allowing researchers to model discrete, pulsatile GH bursts rather than flat, continuous elevation .

📊 Peptide Specifications & Technical Data

Verified via Third-Party HPLC-MS. COA available upon request.

🔬 Primary Research Applications & Investigational Focus

This product is for laboratory research only. The following outlines mechanisms reported in peer-reviewed, non-human studies.

📈 1. Pulsatile GH Secretion Kinetics

The primary utility of CJC-1295 no DAC is modeling endogenous GHRH pulsatility. Unlike long-acting DAC-conjugated analogs which saturate receptors, Mod GRF 1-29's 30-minute half-life allows researchers to study:

• Pulse amplitude modulation
• Somatotroph refractory periods
• Somatostatin inter-pulse antagonism .

🧬 2. GHRH Receptor Binding & Signal Transduction

This peptide is a standard tool for GHRHR binding assays and cAMP response element (CRE) reporter systems. Its high specificity and resistance to DPP-4 degradation make it superior to native GHRH(1-44) for in vitro kinetic studies .

⚖️ 3. GH-IGF-1 Axis Feedback Loops

Researchers utilize Mod GRF 1-29 to investigate negative feedback by IGF-1 on pituitary somatotrophs and hypothalamic GHRH neurons. The short half-life allows precise temporal dissection of feedback inhibition .

🔬 4. Dual-Agonist Synergy (GHRH + GHRP)

CJC-1295 no DAC is the preferred GHRH analog for combination studies with GHRPs (Ipamorelin, GHRP-2, Hexarelin). Its pulsatile profile prevents GHSR-1a desensitization, maximizing synergistic GH release in co-stimulation models .

🧴 5. Dermal & Connective Tissue Fibroblast Models

Emerging research suggests GHRH analogs may exert extra-pituitary effects, including modulation of dermal fibroblast proliferation and collagen synthesis. Mod GRF 1-29 is used to investigate GHRHR expression in peripheral tissues .

🧪 Quality Verification: HPLC, Mass Spectrometry & COA

We do not rely on spot-checking. Every batch of our CJC-1295 no DAC undergoes rigorous analytical chemistry validation per USP/EP guidelines .

1. High Performance Liquid Chromatography (HPLC):

   • Method: Reverse-Phase C18 Column, Gradient Elution.
   • Criteria: Purity ≥99% . Single principal peak with retention time matching reference standard.
   • Scrutiny: Absence of deletion sequences (incomplete synthesis) and oxidation products (Met→Leu substitution prevents oxidation) .

2. Mass Spectrometry (MS):

   • Method: Electrospray Ionization (ESI-MS).
   • Criteria: 3367.97 ± 3.0 Da. Confirms full-length, correctly folded 29-amino acid chain .

3. Endotoxin Analysis (LAL Test):

   • Method: Kinetic Chromogenic LAL.
   • Criteria: <0.05 EU/mg. Significantly exceeds industry standard (≤5 EU/mg), ensuring compatibility with sensitive primary pituitary cell cultures .

4. Certificate of Analysis (COA):
   A batch-specific COA is available immediately upon request. This document includes:

   • Lot Number & Manufacturing Date
   • Retention Time & Purity Percentage
   • MS Spectrum & Molecular Weight Confirmation
   • Endotoxin Value & Appearance Verification

❄️ Handling, Storage & Reconstitution Protocol

For Laboratory Use Only – Not for Human Consumption.

📦 Long-Term Storage (Lyophilized)

• Temperature: Store at -20°C (-4°F) in a manual-defrost freezer .
• Stability: Lyophilized powder is stable for 12 months at -20°C. Stable at room temperature for up to 90 days, but refrigeration is recommended for archival storage .
• Environment: Keep desiccated. Protect from UV light and humidity.

💧 Reconstitution Instructions (Standard Laboratory Protocol)

Based on standard research protocols for GHRH analogs:

1. Equilibrate: Allow the vial to reach room temperature in a desiccator to prevent moisture condensation.
2. Inject: Using a sterile syringe, inject 2.0 mL of Bacteriostatic Water (BAC) slowly against the inner glass wall.
   • Resulting Concentration: 2.5 mg/mL .
3. Swirl: Do NOT vortex or shake vigorously. Shaking introduces oxygen radicals and shears the peptide bonds. Gently swirl until the liquid is clear .

🧊 Post-Reconstitution Handling

• Storage: 2-8°C (Refrigerator). Do not freeze.
• Duration: Use within 21-28 days. Discard if cloudiness or particulate matter persists.
• ⚠️ Critical Alert: Freezing reconstituted peptides causes ice crystal shearing, permanently destroying the tertiary structure. Do not refreeze .

📦 Packaging, Shipping & Buyer Services

We engineer the unboxing experience to match the precision of our analytics.

• 📐 Discreet & Secure Packaging: Vials are seated in medical-grade foam inside rigid, opaque mailers.
• 🌡️ Thermal Integrity: Seasonal cold-pack inserts included to maintain ≤4°C during summer transit .
• 🇺🇸 USA Dispatch: Shipped from domestic logistics hubs. Transit time: 2-5 business days.
• 🛡️ Buyer Guarantees:
  • Authenticity Guarantee: Direct sourcing from cGMP-compliant, FDA-registered facilities.
  • 24/7 Technical Consultation: Lab handling queries answered via email support.
  • Product Replacement: Immediate replacement for damaged or compromised vials (photographic proof required).
  • Complaint Handling: Dedicated resolution team for shipping discrepancies.

❓ Frequently Asked Questions (FAQ)

Q: What is the difference between CJC-1295 "with DAC" and "no DAC"?

A: CJC-1295 with DAC contains a Drug Affinity Complex that binds to albumin, extending the half-life to ~8 days. CJC-1295 no DAC (Mod GRF 1-29) has a short half-life of ~30 minutes. Choose no DAC for pulsatile physiology modeling; choose with DAC for sustained exposure models .

Q: Is Mod GRF 1-29 the same as Sermorelin?

A: No. Sermorelin (GRF 1-29) is the unmodified 29-amino acid sequence with a half-life of <10 minutes. Mod GRF 1-29 is tetrasubstituted (D-Ala², Gln⁸, Ala¹⁵, Leu²⁷) to resist DPP-4 degradation, resulting in a 30-minute half-life. It is a second-generation analog .

Q: What is the purity of this peptide?

A: ≥99% . This is verified via HPLC (area normalization) and ESI-MS for each batch. We do not sell "≥98%" material. Batch COAs are available .

Q: Why is the CAS number sometimes listed as 446036-97-1?

A: 863288-34-0 is the primary CAS number for the no DAC variant. 446036-97-1 is an alternate registry number for related GHRH analogs. We standardize to 863288-34-0 for clarity and regulatory reference .

Q: How do I verify the peptide is correctly synthesized?

A: Check the MS spectrum on the COA. The molecular ion peak should be 3367.97 ± 3.0 Da. Any significant deviation indicates truncation or failed synthesis .

Q: Do you provide dosing instructions?

A: Absolutely not. As a Research Use Only (RUO) supplier, we strictly prohibit providing protocols for human or animal administration. We supply the raw material; determining molar concentrations for specific in vitro assays (e.g., 10nM vs. 100nM) is the responsibility of the qualified researcher .

⚖️ Legal & Compliance Disclaimer

Research Use Only (RUO)
This product is sold exclusively for laboratory research and educational purposes. It is NOT a drug, food, dietary supplement, or cosmetic. This product has not been approved by the U.S. Food and Drug Administration (FDA) . Bodily introduction of any kind, including human or veterinary consumption, injection, or topical application, is strictly prohibited and illegal.

By purchasing from Buy Peptides Online USA, you certify that you are a qualified researcher, scientist, or laboratory professional and will handle this material in compliance with all applicable local, state, and federal regulations, including institutional biosafety protocols .

⭐ Transactional Close: Why Principal Investigators Buy Here

Ready to eliminate confounding variables from your GHRH receptor signaling assays? Here is why top labs buy peptides online from Buy Peptides Online USA:

• 💰 Superior Value: 5mg/vial of ≥99% CJC-1295 no DAC at competitive pricing.
• ⚡ Velocity: Same-day processing on orders placed before 2:00 PM EST.
• 🔒 Secure Checkout: Accepting major credit cards and cryptocurrency (BTC/ETH/USDT) for institutional privacy and discretion.
• 📞 Post-Sale Support: Direct access for technical consultation regarding solubility, storage stability, or analytical data interpretation.

📄 Request a COA: Use our contact form with your Order Number or Batch Lot Number to receive the full analytical package, including HPLC chromatograms and MS spectra.