CJC-1295 (no DAC), Hexarelin (Blend)

🔬 Introduction: The Pulsatile Signal Duo

Welcome to Buy Peptides Online USA, the trusted source for high-purity research peptides online. Our CJC-1295 (no DAC), Hexarelin Blend is a precision-formulated research tool designed for laboratories investigating the hypothalamic-pituitary-somatotropic (HPS) axis.

Unlike single-agent GHRH analogs or standalone GHRPs, this blend allows researchers to model dual-receptor agonism:

1. CJC-1295 (no DAC): A GHRH analog (Growth Hormone-Releasing Hormone) that binds to the GHRH receptor on pituitary somatotrophs, stimulating GH synthesis via the cAMP/PKA/CREB pathway .
2. Hexarelin: A potent GHRP (Growth Hormone-Releasing Peptide) that acts as an agonist at the ghrelin receptor (GHSR-1a) . It amplifies the GH pulse and antagonizes somatostatin tone .

Why no DAC? The absence of the Drug Affinity Complex ensures a short, pulsatile half-life, allowing researchers to study naturalistic secretory bursts rather than continuous receptor saturation. This is critical for modeling negative feedback loops and receptor desensitization kinetics .

This product is strictly for in vitro and laboratory research use only.

✅ Why Choose This CJC/Hexarelin Blend from Buy Peptides Online USA?

The peptide research market is saturated with undefined “research liquids” and single-isolate powders. We differentiate through forensic transparency and synergistic formulation.

Why does this matter? In somatotroph research, impurities and incorrect molar ratios introduce confounding variables that invalidate GH ELISA assays and receptor binding studies. Our blend ensures your data reflects the biology, not the synthesis errors.

🧪 What Is the CJC-1295 (no DAC) & Hexarelin Blend? (Mechanistic Deep-Dive)

🔹 Component A: CJC-1295 (no DAC) – The GHRH Analog

• Full Sequence (Single Letter): YaDAIFTQSYRKVLAQLSARKLLQDILSR-NH₂
• Full Sequence (Triple Letter): Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH₂
• Molecular Formula: C₁₅₂H₂₅₂N₄₄O₄₂
• Molecular Weight: ~3367.9 g/mol
• CAS Number: 446036-97-1 (Primary) / 863288-34-0 (Alternate)
• Synonyms: Modified GRF(1-29), GHRH(1-29)-NH₂, Tetracosactide-related peptide

🔬 Mechanism of Action:
CJC-1295 (no DAC) is a 29-amino acid peptide derived from the bioactive core of human GHRH. It features a D-Ala substitution at position 2, which confers resistance to dipeptidyl peptidase-4 (DPP-4) degradation . Upon binding the GHRH receptor, it activates adenylyl cyclase, elevating intracellular cAMP. This triggers Protein Kinase A (PKA) , leading to phosphorylation of CREB and subsequent transcription of the GH1 gene .

⏳ Half-Life Profile (No DAC): Approximately 30–60 minutes. This short half-life is intentional for researchers modeling physiological pulsatility .

🔹 Component B: Hexarelin – The Super GHRP

• Sequence: His-D-2-Me-Trp-Ala-Trp-D-Phe-Lys-NH₂
• Molecular Formula: C₄₇H₅₇N₁₁O₆
• Molecular Weight: ~887.0 g/mol
• CAS Number: 140703-51-1
• Synonyms: Examorelin, MR-256, GHRP-6 Super Analog

🔬 Mechanism of Action:
Hexarelin is a synthetic hexapeptide and a potent agonist at the Growth Hormone Secretagogue Receptor type 1a (GHSR-1a) . Unlike GHRP-6, Hexarelin contains a D-2-methyltryptophan residue, making it highly resistant to enzymatic cleavage. This results in superior GH secretory potency compared to first-generation GHRPs . It operates via the PLC/IP3/PKC pathway, increasing intracellular calcium flux.

⚡ Synergy Rationale:
When combined, GHRH + GHRP produce synergistic GH release—significantly greater than the sum of their individual effects. This is due to convergent signaling on the somatotroph: cAMP/PKA (CJC) sensitizes the cell, while Ca²⁺ flux (Hexarelin) triggers vesicle exocytosis.

📊 Peptide Specifications & Technical Data

Verified via Third-Party HPLC-MS. COA available upon request.

🔬 Primary Research Applications & Investigational Focus

This product is for laboratory research only. The following outlines mechanisms reported in peer-reviewed, non-human studies.

📈 1. Pulsatile GH Secretion Kinetics

The primary utility of this blend is modeling endogenous GH pulsatility. Unlike CJC-1295 with DAC (half-life ~8 days), the no-DAC variant allows researchers to simulate fast-onset, fast-clearance pulses. Hexarelin’s potency ensures a measurable GH spike within minutes of receptor activation .

🧠 2. Somatotroph Desensitization & Feedback

Chronic exposure to GHRH analogs can downregulate the GHRH receptor. This blend is optimal for time-course studies investigating receptor internalization, somatostatin feedback, and IGF-1 negative inhibition .

⚖️ 3. GHSR-1a Signaling Cascades

Hexarelin is a superior tool for mapping the GHSR-1a interactome. Researchers use this blend to study β-arrestin recruitment, GRK phosphorylation, and receptor recycling kinetics .

💪 4. Metabolic & Anabolic Modeling

GH release stimulates hepatic IGF-1 production. This blend is utilized in rodent models to study lean mass retention, lipolysis, and nitrogen retention during caloric restriction.

🧪 Quality Verification: HPLC, Mass Spectrometry & COA

We do not rely on spot-checking. Every batch of our CJC-1295 (no DAC), Hexarelin Blend undergoes rigorous analytical chemistry validation .

1. High Performance Liquid Chromatography (HPLC): Confirms ≥99% absolute purity. Chromatograms are scrutinized for deletion sequences and incomplete synthesis. Retention time is matched against certified reference standards .
2. Mass Spectrometry (MS): Verifies exact molecular weight (3367.9 Da and 887.0 Da). This confirms the peptide chain is full-length and correctly folded .
3. Certificate of Analysis (COA): A batch-specific COA is available immediately upon request. This document includes the lot number, retention time, purity percentage, and assay date.

❄️ Handling, Storage & Reconstitution Protocol

For Laboratory Use Only – Not for Human Consumption.

📦 Long-Term Storage (Lyophilized)

• Temperature: Store at -20°C (-4°F) in a manual-defrost freezer.
• Environment: Keep desiccated. Protect from UV light and humidity .
• Stability: Stable for 12 months under these conditions .

💧 Reconstitution Instructions (Standard Laboratory Protocol)

Based on standard research protocols for GHRH/GHRP blends :

1. Equilibrate: Allow the vial to reach room temperature in a desiccator to prevent condensation.
2. Inject: Using a sterile syringe, inject 2.0 mL of Bacteriostatic Water (BAC) slowly against the inner glass wall.
   • Resulting Concentration: 5 mg/mL total peptide (2.5 mg/mL CJC + 2.5 mg/mL Hexarelin).
3. Swirl: Do NOT vortex or shake vigorously. Shaking introduces oxygen radicals and shears the peptide bonds. Gently swirl until the liquid is clear .

🧊 Post-Reconstitution Handling

• Storage: 2-8°C (Refrigerator).
• Duration: Use within 21-28 days. Discard if cloudiness persists .
• ⚠️ Critical Alert: Freezing reconstituted peptides causes ice crystal shearing, permanently destroying the tertiary structure. Do not refreeze .

📦 Packaging, Shipping & Buyer Services

We engineer the unboxing experience to match the precision of our analytics.

• 📐 Discreet & Secure Packaging: Vials are seated in medical-grade foam inside rigid, opaque mailers.
• 🌡️ Thermal Integrity: Seasonal cold-pack inserts included to maintain ≤4°C during summer transit.
• 🇺🇸 USA Dispatch: Shipped from domestic logistics hubs. Transit time: 2-5 business days.
• 🛡️ Buyer Guarantees:
  • Authenticity Guarantee: Direct sourcing from cGMP-compliant, FDA-registered facilities.
  • 24/7 Technical Consultation: Lab handling queries answered via email support.
  • Product Replacement: Immediate replacement for damaged or compromised vials (photographic proof required).
  • Complaint Handling: Dedicated resolution team for shipping discrepancies.

❓ Frequently Asked Questions (FAQ)

Q: What is the difference between CJC-1295 “with DAC” and “no DAC”?

A: CJC-1295 with DAC contains a Drug Affinity Complex that binds to albumin, extending the half-life to ~8 days. CJC-1295 no DAC has a short half-life (~30 min), allowing researchers to model physiological GH pulses rather than continuous receptor activation .

Q: Why blend Hexarelin with CJC instead of GHRP-2 or Ipamorelin?

A: Hexarelin is significantly more potent than GHRP-2 or Ipamorelin. It is the preferred GHRP for researchers requiring maximal GH secretory amplitude in acute dosing studies. Its D-2-methyltryptophan modification grants superior enzymatic stability .

Q: Is the purity really ≥99%?

A: Yes. This is not an averaged value. Each component is independently synthesized, purified, and verified via HPLC-MS to meet the ≥99% threshold prior to blending .

Q: Can I mix this blend with other GHRPs in the same syringe?

A: For laboratory research only, peptides can be drawn into the same syringe immediately prior to assay administration. However, pre-mixing in the vial is not recommended for long-term storage, as dilution ratios and preservative efficacy may be compromised .

Q: How long does the peptide last after reconstitution?

A: When reconstituted with Bacteriostatic Water (0.9% benzyl alcohol), the solution is stable for 21-30 days at 2-8°C. Sterile water without preservatives shortens this window significantly .

Q: Do you provide dosing instructions?

A: Absolutely not. As a Research Use Only (RUO) supplier, we strictly prohibit providing protocols for human or animal administration. We supply the raw material; determining molar concentrations for specific in vitro assays (e.g., 10nM vs. 100nM) is the responsibility of the qualified researcher.

⚖️ Legal & Compliance Disclaimer

Research Use Only (RUO)
This product is sold exclusively for laboratory research and educational purposes . It is NOT a drug, food, dietary supplement, or cosmetic. This product has not been approved by the U.S. Food and Drug Administration (FDA) . Bodily introduction of any kind, including human or veterinary consumption, injection, or topical application, is strictly prohibited and illegal.

By purchasing from Buy Peptides Online USA, you certify that you are a qualified researcher, scientist, or laboratory professional and will handle this material in compliance with all applicable local, state, and federal regulations, including institutional biosafety protocols.

⭐ Transactional Close: Why Principal Investigators Buy Here

Ready to eliminate confounding variables from your somatotroph signaling assays? Here is why top labs buy peptides online from Buy Peptides Online USA:

• 💰 Superior Value: 10mg total peptide mass (5mg/5mg) at a competitive price point versus buying individual 5mg vials separately.
• ⚡ Velocity: Same-day processing on orders placed before 2:00 PM EST.
• 🔒 Secure Checkout: Accepting major credit cards and cryptocurrency (BTC/ETH/USDT) for institutional privacy and discretion.
• 📞 Post-Sale Support: Direct access for technical consultation regarding solubility, storage stability, or analytical data interpretation.

📄 Request a COA: Use our contact form with your Order Number or Batch Lot Number to receive the full analytical package, including HPLC chromatograms and MS spectra.