BPC-157, TB-500, KPV, GHK-Cu (Klow Blend)

🔬 Introduction: The Quadruple-Pathway Research Standard

Welcome to Buy Peptides Online USA, the trusted source for high-purity research peptides online. Our Klow Blend is not merely a collection of peptides; it is a precision-formulated research tool designed for laboratories investigating the intersection of inflammation, extracellular matrix (ECM) remodeling, and cytoskeletal repair.

While standard “Glow” blends target angiogenesis and dermal architecture, Klow introduces a fourth, critical variable: KPV (Lys-Pro-Val) . This α-MSH fragment allows researchers to model the direct inhibition of NF-κB signaling simultaneously with the regenerative actions of BPC-157, TB-500, and GHK-Cu .

This blend is optimized for:

1. ⬇️ Anti-Cytokine Signaling: Direct modulation of IL-1β, TNF-α, and COX-2 expression.
2. 🩸 Angiogenesis & Perfusion: VEGF/eNOS upregulation via BPC-157.
3. 🧬 ECM Turnover & Collagen Deposition: Copper-mediated MMP regulation and fibroblast activation.
4. 🚀 Cytoskeletal Dynamics: Actin polymerization and cell migration via TB-500.

This product is strictly for in vitro and laboratory research use only.

✅ Why Choose This Klow Blend from Buy Peptides Online USA?

The peptide research market is saturated with vague “research liquids” and untested powders. We differentiate through forensic transparency.

Why does this matter? In peptide research, truncated sequences and elevated endotoxins are confounding variables that destroy assay reproducibility. Our Klow Blend is synthesized to peptide therapeutic-grade specifications, ensuring your data reflects the biology, not the impurities .

🧪 What Is the Klow Blend? (Component Deep-Dive)

The Klow Blend distinguishes itself from the “Glow Blend” by the inclusion of KPV. This creates a unique Quadrant Model for regenerative research .

🔹 Component A: BPC-157 (10mg) – The Cytoprotective Chaperone

• Full Sequence: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val
• Molecular Formula: C₆₂H₉₈N₁₆O₂₂
• Molecular Weight: ~1419.5 g/mol
• CAS Number: 137525-51-0
• Research Role: Upregulation of VEGFR-2 and Akt. Promotes fibroblast survival under hypoxic conditions. Modulates the nitric oxide (NO) system to protect endothelial integrity .

🔹 Component B: TB-500 (10mg) – The Actin Chaperone

• Synonym: Thymosin Beta-4 Fragment (43aa)
• Molecular Formula: C₂₁₂H₃₅₀N₅₆O₇₈S
• Molecular Weight: ~4963.44 g/mol
• CAS Number: 77591-33-4
• Research Role: Sequesters G-actin, increasing the monomeric pool required for rapid F-actin polymerization. Essential for lamellipodia extension and scratch assay closure rates .

🔹 Component C: KPV (10mg) – The Anti-Inflammatory Tripeptide

• Sequence: Lys–Pro–Val (α-MSH 11-13 Fragment)
• Molecular Formula: C₁₆H₃₀N₄O₄
• Molecular Weight: ~342.43 g/mol
• CAS Number: 67727-97-3
• PubChem CID: 125672
• Research Role: This is the differentiator. KPV is a potent inhibitor of NF-κB nuclear translocation. Unlike general anti-inflammatories, KPV acts directly on immune signaling cascades without melanotropic effects. It is transported via the PepT1 transporter in intestinal models .

🔹 Component D: GHK-Cu (50mg) – The Matrix Modulator

• Sequence: Gly–His–Lys (Copper Chelate)
• Molecular Formula: C₁₄H₂₂CuN₆O₄
• Molecular Weight: ~401.91 g/mol (Copper-bound)
• CAS Number: 89030-95-5
• Research Role: Upregulation of decorin and glycosaminoglycans. Activates NRF2/ARE antioxidant pathways. Visual confirmation of copper chelation is evident via the characteristic blue tint of the lyophilized cake .

📊 Peptide Specifications & Technical Data

Verified via Third-Party HPLC-MS. COA available upon request.

*Critical Visual QC: The presence of a faint blue hue in the lyophilized cake/solution is a positive quality indicator for GHK-Cu, confirming active copper chelation .*

🔬 Primary Research Applications & Investigational Focus

This product is for laboratory research only. The following outlines mechanisms reported in peer-reviewed, non-human studies.

🛡️ 1. Inflammatory Cytokine Suppression (KPV-Dominant)

Standard regenerative blends lack a dedicated anti-inflammatory agent. KPV fills this gap. Researchers utilize this blend to study the dual-action of repair + immune quiescence. KPV has been shown in preclinical models to inhibit IL-1β, TNF-α, and IL-6 secretion in LPS-stimulated macrophages .

🧵 2. ECM Homeostasis & Fibroblast Senescence

GHK-Cu is a potent modulator of matrix metalloproteinases (MMPs) . When combined with TB-500 (cell migration) and BPC-157 (cell survival), this blend offers a robust model for studying dermal fibroblast senescence and collagen cross-linking kinetics .

🩸 3. Angiogenesis in Inflamed Microenvironments

Standard angiogenesis assays use healthy endothelial cells. This blend allows researchers to model angiogenesis under inflammatory duress—a more accurate representation of chronic wound and diabetic ulcer models .

💪 4. Skeletal Muscle & Fascial Integrity

TB-500 and BPC-157 are extensively cited for their synergistic roles in myoblast proliferation and tendon fibroblast linear migration. The addition of KPV helps parse the role of prostaglandin signaling in muscle adaptation versus repair .

🧪 Quality Verification: 5X Testing Protocol

We do not rely on spot-checking. Every batch of our Klow Blend undergoes pentavalent analysis .

1. High Performance Liquid Chromatography (HPLC): Confirms ≥99% absolute purity. Chromatograms are scrutinized for deletion sequences and incomplete synthesis.
2. Mass Spectrometry (MS): Verifies exact molecular weight of all four components (1419.5 Da, 4963.44 Da, 342.43 Da, 401.91 Da).
3. Endotoxin Analysis (LAL Test): Verified <0.05 EU/mg. Critical for sensitive macrophage and dendritic cell cultures.
4. Stability Assay: Accelerated degradation studies confirm 12-month shelf stability at -20°C.
5. Visual/Appearance Inspection: Validates lyophilized cake integrity and correct GHK-Cu chromatic signature.

Certificate of Analysis (COA): A batch-specific COA is available immediately upon request. This document includes the lot number, retention time, purity percentage, and assay date.

❄️ Handling, Storage & Reconstitution Protocol

For Laboratory Use Only – Not for Human Consumption.

📦 Long-Term Storage (Lyophilized)

• Temperature: Store at -20°C (-4°F) in a manual-defrost freezer.
• Environment: Keep desiccated. Protect from UV light and humidity.
• Stability: Stable for 12 months under these conditions.

💧 Reconstitution Instructions (Standard Laboratory Protocol)

Based on standard 80mg vial protocols :

1. Equilibrate: Allow the vial to reach room temperature in a desiccator to prevent condensation.
2. Inject: Using a sterile syringe, inject 3.0 mL of Bacteriostatic Water (BAC) slowly against the inner glass wall.
   • Resulting Concentration: ~26.7 mg/mL total peptide.
   • Individual Concentrations: BPC-157/TB-500/KPV = ~3.33 mg/mL each. GHK-Cu = ~16.7 mg/mL.
3. Swirl: Do NOT vortex or shake vigorously. Shaking introduces oxygen radicals and shears the peptide bonds. Gently swirl until the liquid is clear/lightly opalescent.
   • Note: GHK-Cu solutions will appear faint blue. This is normal and expected .

🧊 Post-Reconstitution Handling

• Storage: 2-8°C (Refrigerator).
• Duration: Use within 21-28 days. Discard if cloudiness persists.
• ⚠️ Critical Alert: Freezing reconstituted peptides causes ice crystal shearing, permanently destroying the tertiary structure. Do not refreeze.

📦 Packaging, Shipping & Buyer Services

We engineer the unboxing experience to match the precision of our analytics.

• 📐 Discreet & Secure Packaging: Vials are seated in medical-grade foam inside rigid, opaque mailers.
• 🌡️ Thermal Integrity: Seasonal cold-pack inserts included to maintain ≤4°C during summer transit.
• 🇺🇸 USA Dispatch: Shipped from domestic logistics hubs. Transit time: 2-5 business days.
• 🛡️ Buyer Guarantees:
  • Authenticity Guarantee: Direct sourcing from cGMP-compliant, FDA-registered facilities.
  • 24/7 Technical Consultation: Lab handling queries answered via email support.
  • Product Replacement: Immediate replacement for damaged or compromised vials (photographic proof required).
  • Complaint Handling: Dedicated resolution team for shipping discrepancies.

❓ Frequently Asked Questions (FAQ)

Q: What is the difference between “Glow” and “Klow”?

A: Glow Blend typically contains BPC-157, TB-500, and GHK-Cu. Klow Blend adds KPV (Lys-Pro-Val) , a potent anti-inflammatory tripeptide fragment of α-MSH. Klow is therefore the preferred blend for researchers specifically modeling inflammation-resolution dynamics .

Q: Is the purity really ≥99% for all four components?

A: Yes. This is not an averaged value. Each component (BPC-157, TB-500, KPV, GHK-Cu) is independently synthesized, purified, and verified via HPLC-MS to meet the ≥99% threshold prior to blending. Batch COAs are available .

Q: Why is the GHK-Cu quantity 50mg while the others are 10mg?

A: This is the standardized research ratio. GHK-Cu is typically studied at 5x the molar concentration of the other peptides due to its role as an enzymatic cofactor (copper donor) rather than a direct signaling ligand .

Q: How long does the peptide last after reconstitution?

A: When reconstituted with Bacteriostatic Water (0.9% benzyl alcohol), the solution is stable for 21-30 days at 2-8°C. Sterile water without preservatives shortens this window significantly .

Q: Do you provide dosing instructions?

A: Absolutely not. As a Research Use Only (RUO) supplier, we strictly prohibit providing protocols for human or animal administration. We supply the raw material; determining molar concentrations for specific in vitro assays (e.g., 10μM vs. 50μM) is the responsibility of the qualified researcher.

⚖️ Legal & Compliance Disclaimer

Research Use Only (RUO)
This product is sold exclusively for laboratory research and educational purposes . It is NOT a drug, food, dietary supplement, or cosmetic. This product has not been approved by the U.S. Food and Drug Administration (FDA) . Bodily introduction of any kind, including human or veterinary consumption, injection, or topical application, is strictly prohibited and illegal.

By purchasing from Buy Peptides Online USA, you certify that you are a qualified researcher, scientist, or laboratory professional and will handle this material in compliance with all applicable local, state, and federal regulations, including institutional biosafety protocols.

⭐ Transactional Close: Why Principal Investigators Buy Here

Ready to eliminate confounding variables from your tissue remodeling assays? Here is why top labs buy peptides online from Buy Peptides Online USA:

• 💰 Superior Value: 80mg total peptide mass (including 50mg GHK-Cu) at a competitive price point versus buying 5mg vials individually.
• ⚡ Velocity: Same-day processing on orders placed before 2:00 PM EST.
• 🔒 Secure Checkout: Accepting major credit cards and cryptocurrency (BTC/ETH/USDT) for institutional privacy and discretion.
• 📞 Post-Sale Support: Direct access for technical consultation regarding solubility, storage stability, or analytical data interpretation.

📄 Request a COA: Use our contact form with your Order Number or Batch Lot Number to receive the full analytical package, including HPLC chromatograms and MS spectra.